Gego Audrey, Silvie Olivier, Franetich Jean-François, Farhati Khemaïs, Hannoun Laurent, Luty Adrian J F, Sauerwein Robert W, Boucheix Claude, Rubinstein Eric, Mazier Dominique
Inserm U511, F-75013 Paris, France.
Antimicrob Agents Chemother. 2006 Apr;50(4):1586-9. doi: 10.1128/AAC.50.4.1586-1589.2006.
Plasmodium liver stages represent potential targets for antimalarial prophylactic drugs. Nevertheless, there is a lack of molecules active on these stages. We have now developed a new approach for the high-throughput screening of drug activity on Plasmodium liver stages in vitro, based on an infrared fluorescence scanning system. This method allowed us to count automatically and rapidly Plasmodium-infected hepatocytes, using different hepatic cells and different Plasmodium species, including Plasmodium falciparum. This new technique is well adapted for high-throughput drug screening and should facilitate the identification of new antimalarial compounds active on Plasmodium liver stages.
疟原虫肝期是抗疟预防性药物的潜在靶点。然而，目前缺乏对这些阶段有活性的分子。我们现已开发出一种基于红外荧光扫描系统的新方法，用于在体外高通量筛选针对疟原虫肝期的药物活性。该方法使我们能够利用不同的肝细胞和不同的疟原虫种类（包括恶性疟原虫）自动且快速地计数被疟原虫感染的肝细胞。这项新技术非常适合高通量药物筛选，应有助于鉴定对疟原虫肝期有活性的新型抗疟化合物。
