Gravallese E M, Darling J M, Ladd A L, Katz J N, Glimcher L H
Department of Rheumatology and Immunology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Arthritis Rheum. 1991 Sep;34(9):1076-84. doi: 10.1002/art.1780340903.
Destructive joint changes in rheumatoid arthritis (RA) are thought to be mediated in part by the neutral proteinases collagenase and stromelysin. Collagenase messenger RNA (mRNA) has been previously localized to the synovial lining layer. In this study, synovial tissue from 8 patients with RA and 2 patients with osteoarthritis was examined for proteinase production by in situ hybridization. Stromelysin mRNA localized predominantly to the synovial lining layer cells. In serial sections, collagenase mRNA was shown to be localized to the same tissue areas as those producing stromelysin mRNA, and grain counts revealed a direct correlation between production of stromelysin mRNA and production of collagenase mRNA. All patients with RA were producing collagenase and stromelysin mRNA in detectable amounts. One of 2 osteoarthritis patients was producing these metalloproteinases, but in levels below those found in the RA patients. These data support the identity of the synovial lining cells as the major synovial cells producing collagenase and stromelysin in RA and provide new evidence for the coordinate production of collagenase and stromelysin in RA in vivo.
类风湿性关节炎（RA）中的关节破坏改变被认为部分是由中性蛋白酶胶原酶和基质溶素介导的。胶原酶信使核糖核酸（mRNA）先前已定位到滑膜衬里层。在本研究中，通过原位杂交检查了8例RA患者和2例骨关节炎患者的滑膜组织中的蛋白酶产生情况。基质溶素mRNA主要定位于滑膜衬里层细胞。在连续切片中，胶原酶mRNA显示定位于与产生基质溶素mRNA相同的组织区域，并且颗粒计数显示基质溶素mRNA产生与胶原酶mRNA产生之间存在直接相关性。所有RA患者均产生可检测量的胶原酶和基质溶素mRNA。2例骨关节炎患者中有1例产生这些金属蛋白酶，但水平低于RA患者中发现的水平。这些数据支持滑膜衬里细胞作为RA中产生胶原酶和基质溶素的主要滑膜细胞的身份，并为RA体内胶原酶和基质溶素的协同产生提供了新证据。
