Robbins P W
Am J Clin Pathol. 1975 May;63(5):671-6. doi: 10.1093/ajcp/63.5.671.
Transformation of the chick fibroblast surface has been studied in cells infected with Schmidt-Ruppin Rous sarcoma virus and the temperature-sensitive mutant of this virus, TS-68. Major findings following transformation induced by a shift from nonpermissive (41 C.) to permissive (36 C.) temperature in TS-68 infected cells were: (1) rapid cessation or slowing of the synthesis of a protein, M.W. 100-200,000, localization uncertain; (2) cessation or slowing of the synthesis of a plasma membrane protein, M.W. 45,000, within 2-4 hours; (3) cessation or slowing of the synthesis of a large trypsin- and collagenase- sensitive protein (M.W. greater than 200,000) only after an extended period of morphologic transformation. In addition, increased quantities of type-specific viral antigen in the membranes of infected cells were observed in TS-68-infected cells at 41 compared with 36 C.
已在感染施密特 - 鲁平劳氏肉瘤病毒及其温度敏感突变体TS - 68的鸡成纤维细胞中研究了细胞表面的转化。在TS - 68感染的细胞中，从非允许温度（41℃）转变为允许温度（36℃）诱导转化后，主要发现如下：（1）一种分子量为100 - 200,000的蛋白质合成迅速停止或减慢，定位不确定；（2）在2 - 4小时内，一种分子量为45,000的质膜蛋白合成停止或减慢；（3）仅在长时间形态转化后，一种对胰蛋白酶和胶原酶敏感的大蛋白（分子量大于200,000）合成停止或减慢。此外，与36℃相比，在41℃下的TS - 68感染细胞中观察到感染细胞膜中特异性病毒抗原的量增加。
