Shiu R P, Pouyssegur J, Pastan I
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3840-4. doi: 10.1073/pnas.74.9.3840.
Chick embryo fibroblasts transformed by Rous sarcoma virus have an increased content of two membrane proteins of molecular weights 78,000 and 95,000. The increased content of the 95,000-dalton protein and the principal increase in the content of the 78,000-dalton protein are not an early consequence of cell transformation but instead are secondary to the rapid depletion of glucose from the growth medium of transformed cells. When glucose is maintained at high levels in the growth medium of transformed cells, the synthesis of the 95,000-dalton protein is arrested and that of the 78,000-dalton protein is markedly suppressed. Upon removal of glucose from the growth medium of normal cells, these proteins increase to levels comparable to those of transformed cells. Because the amount of these two proteins is influenced by the presence or absence of glucose, we suggest they be referred to as "glucose-regulated proteins." GRP-78 and GRP-95. These proteins may have an important role in regulating the utilization of glucose in cultured cells.
被劳氏肉瘤病毒转化的鸡胚成纤维细胞中，分子量分别为78,000和95,000的两种膜蛋白含量增加。95,000道尔顿蛋白含量的增加以及78,000道尔顿蛋白含量的主要增加并非细胞转化的早期结果，而是继发于转化细胞生长培养基中葡萄糖的快速消耗。当在转化细胞的生长培养基中维持高浓度葡萄糖时，95,000道尔顿蛋白的合成停止，78,000道尔顿蛋白的合成则受到显著抑制。从正常细胞的生长培养基中去除葡萄糖后，这些蛋白的水平会升高至与转化细胞相当的水平。由于这两种蛋白的量受葡萄糖存在与否的影响，我们建议将它们称为“葡萄糖调节蛋白”，即GRP - 78和GRP - 95。这些蛋白可能在调节培养细胞中葡萄糖的利用方面发挥重要作用。
