College of Chemistry and Environmental Science, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Mar;75(3):1013-7. doi: 10.1016/j.saa.2009.12.039. Epub 2009 Dec 16.
A highly sensitive visible spectrophotometric method has been developed to determine ethamsylate in this paper, which is based on using Cu(II) as spectroscopic probe reagent. The study indicates that in the presence of SCN(-) and KNO(3), Cu(II) is reduced to Cu(I) by ethamsylate at pH 5.0, and the in situ formed Cu(I) reacts with SCN(-) to form into the white emulsion CuSCN that could be stayed upon the surface of water. According to the amount of residual Cu(II), the amount of ethamsylate can be indirectly determined. Under the optimal conditions, Beer's law is applicable in the range of 0.2-9.0 microg/mL (7.60x10(-7)-3.42x10(-5)mol/L) for aqueous standard solution of ethamsylate with linear correlation coefficient of 0.9998. The detection limit and relative standard deviation are 0.12 microg/mL and 1.5%, respectively. And the molar absorption coefficient of the indirect determination of ethamsylate is 1.0x10(5)L/mol cm. The method is successfully applied to determine ethamsylate in pharmaceutical preparations and biological samples.
本文提出了一种用 Cu(II) 作为光谱探针试剂测定依他酸的高灵敏度可见分光光度法。研究表明，在 SCN(-) 和 KNO(3)存在下，依他酸在 pH 5.0 下将 Cu(II)还原为 Cu(I)，生成的 Cu(I)与 SCN(-)反应生成白色乳状的 CuSCN，可留在水面上。根据残留的 Cu(II)量，可间接测定依他酸的含量。在最佳条件下，依他酸水溶液标准溶液在 0.2-9.0 μg/mL 范围内（7.60x10(-7)-3.42x10(-5)mol/L）呈比尔定律关系，线性相关系数为 0.9998。检测限和相对标准偏差分别为 0.12 μg/mL 和 1.5%。间接测定依他酸的摩尔吸光系数为 1.0x10(5)L/mol cm。该方法成功应用于药物制剂和生物样品中依他酸的测定。
