Takei Y, Smelser G K
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1975;194(3):153-73. doi: 10.1007/BF00496873.
Adult rabbit zonular fibers maintained in their native condition were treated with collagenase, alpha-chymotrypsin and hyaluronidase, and were observed with the electron microscope. The results obtained were as follows: 1. Collagenase digested the lens capsule, but not the zonular fibers. 2. Long time collagenase action obscured the cell membrane of the lens epithelium and the basal lamina of the ciliary epithelium. 3. Washing with 0.9% NaCl increased the collagenase action on the lens capsule. 4. Alpha-chymotrypsin digested the zonular fibers and the zonular lemalla, but not the lens capsule and the basal lamina of the ciliary epithelium. 5, Hyaluronidase only slightly changed the lens capsule. 6. The vitreous fibers were digested by collagenase, but not by alpha-chymotrypsin or hyaluronidase. Thes results together with the review of recent literature indicate that the zonular fiber has a nature close to that of the microfibril of elastic fiber.
对处于天然状态的成年兔晶状体悬韧带纤维用胶原酶、α-糜蛋白酶和透明质酸酶进行处理，并用电镜观察。得到的结果如下：1. 胶原酶能消化晶状体囊，但不能消化悬韧带纤维。2. 长时间作用的胶原酶会使晶状体上皮细胞膜和睫状上皮基膜模糊不清。3. 用0.9%氯化钠冲洗可增强胶原酶对晶状体囊的作用。4. α-糜蛋白酶能消化悬韧带纤维和悬韧带薄片，但不能消化晶状体囊和睫状上皮基膜。5. 透明质酸酶仅使晶状体囊稍有变化。6. 玻璃体纤维可被胶原酶消化，但不能被α-糜蛋白酶或透明质酸酶消化。这些结果以及对近期文献的回顾表明，悬韧带纤维的性质与弹性纤维微原纤维的性质相近。
