Cheppudira Bopaiah Pooviah
Department of Anesthesiology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
J Neurosci Methods. 2006 Jul 15;155(1):122-5. doi: 10.1016/j.jneumeth.2006.01.001. Epub 2006 Mar 29.
The paw withdrawal latency to thermal radiant heat stimuli is a widely used nociceptive measure to study hyperalgesic mechanisms. In the present study, in addition to the paw withdrawal latency, two behavioral components of pain behaviors, paw licking and paw lifting have been characterized and quantified. The thermal stimuli were successively applied to the plantar surface of the rat hind paws and recorded the behavioral responses to each of the stimuli. Noxious radiant heat significantly increased the frequency of paw lifting behavior in naïve rats. On the other hand, the thermal stimuli significantly evoked the occurrence of paw licking behavior in inflamed paws and it was maintained at all time points of measurements. The paw withdrawal latency decreased in inflamed rats in comparison with control rats. These data informs that noxious radiant heat specifically evokes the frequency of paw lifting behavior in normal physiological condition, and paw licking behavior in a pathological inflammatory condition. These findings suggest that in addition to the measurement of PWL, scoring of paw licking and lifting behaviors will improve the sensitivity of this pain test.
爪对热辐射热刺激的缩爪潜伏期是一种广泛用于研究痛觉过敏机制的伤害性测量方法。在本研究中，除了缩爪潜伏期外，还对疼痛行为的两个行为成分，即舔爪和举爪进行了特征描述和量化。将热刺激依次施加于大鼠后爪的足底表面，并记录对每种刺激的行为反应。有害的辐射热显著增加了未处理大鼠的举爪行为频率。另一方面，热刺激显著诱发了发炎爪子的舔爪行为，并且在所有测量时间点都持续存在。与对照大鼠相比，发炎大鼠的缩爪潜伏期缩短。这些数据表明，有害的辐射热在正常生理条件下特异性地诱发举爪行为的频率，而在病理性炎症条件下诱发舔爪行为。这些发现表明，除了测量缩爪潜伏期外，对舔爪和举爪行为进行评分将提高这种疼痛测试的敏感性。
