Bai Zhan-Tao, Liu Tong, Chai Zhi-Fang, Pang Xue-Yan, Ji Yong-Hua
Graduate School of the Chinese Academy of Sciences, Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
Eur J Pharmacol. 2006 Dec 15;552(1-3):67-77. doi: 10.1016/j.ejphar.2006.09.018. Epub 2006 Sep 23.
The developmental and pharmacological characteristics of pain responses induced by the experimental scorpion BmK (Buthus martensi Karsch) sting were detailed in this study. Following the unilateral intraplantar injection of BmK venom into rat hind paw, it was found: 1) BmK venom induced an edematogenic response, spontaneous pain and pain hypersensitivity in a dose-dependent manner; 2) the paw edema and flare were induced rapidly and restricted at the injected paw for about 24-48 h; 3) the monophasic tonic spontaneous pain manifested as continuous paw flinching and lifting/licking of the injected paw and lasted for more than 2 h; 4) the detectable thermal hypersensitivity to radiant heat stimuli was just at the injected side for about 72-96 h; 5) the mechanical hypersensitivity to von Frey filaments was evoked surprisingly to be the bilateral and mirror-like for about 2-3 weeks; 6) morphine, indomethacin and bupivacaine could suppress BmK venom-induced pain responses with different intensity and time courses. The results indicated that the experimental BmK sting could evoke the prolonged paw inflammation, tonic spontaneous behaviors, unilateral thermal and bilateral mechanical hypersensitivity. The distinct time development of pain responses induced by experimental BmK sting might be involved in different nervous and/or tissue mechanisms. The experimental BmK sting test thus may be an available tissue injury-induced tonic inflammatory pain model for understanding the mechanisms underlying clinical spontaneous pain, thermal and mirror-imaged bilateral mechanical pain hypersensitivity.
本研究详细阐述了实验性东亚钳蝎（Buthus martensi Karsch）蛰伤诱导的疼痛反应的发育和药理学特征。在大鼠后爪单侧足底注射东亚钳蝎毒液后，发现：1）东亚钳蝎毒液以剂量依赖性方式诱导致水肿反应、自发痛和痛觉过敏；2）爪部水肿和红斑迅速出现，并局限于注射爪部约24 - 48小时；3）单相强直性自发痛表现为注射爪部持续的爪部退缩和抬起/舔舐，持续超过2小时；4）对辐射热刺激的可检测热超敏反应仅在注射侧持续约72 - 96小时；5）对von Frey细丝的机械超敏反应令人惊讶地在双侧呈镜像出现，持续约2 - 3周；6）吗啡、吲哚美辛和布比卡因可不同程度和时间进程地抑制东亚钳蝎毒液诱导的疼痛反应。结果表明，实验性东亚钳蝎蛰伤可诱发长时间的爪部炎症、强直性自发行为、单侧热超敏和双侧机械超敏。实验性东亚钳蝎蛰伤诱导的疼痛反应的不同时间发展可能涉及不同的神经和/或组织机制。因此，实验性东亚钳蝎蛰伤试验可能是一种有用的组织损伤诱导的强直性炎症性疼痛模型，用于理解临床自发痛、热痛和镜像双侧机械性疼痛超敏的潜在机制。
