Reece R J, Maxwell A
Department of Biochemistry, University of Leicester, UK.
Crit Rev Biochem Mol Biol. 1991;26(3-4):335-75. doi: 10.3109/10409239109114072.
DNA gyrase is an essential bacterial enzyme that catalyzes the ATP-dependent negative super-coiling of double-stranded closed-circular DNA. Gyrase belongs to a class of enzymes known as topoisomerases that are involved in the control of topological transitions of DNA. The mechanism by which gyrase is able to influence the topological state of DNA molecules is of inherent interest from an enzymological standpoint. In addition, much attention has been focused on DNA gyrase as the intracellular target of a number of antibacterial agents as a paradigm for other DNA topoisomerases. In this review we summarize the current knowledge concerning DNA gyrase by addressing a wide range of aspects of the study of this enzyme.
DNA促旋酶是一种必需的细菌酶，可催化双链闭环DNA的ATP依赖性负超螺旋。促旋酶属于一类称为拓扑异构酶的酶，参与DNA拓扑转变的控制。从酶学角度来看，促旋酶影响DNA分子拓扑状态的机制本身就很有趣。此外，作为许多抗菌剂的细胞内靶点以及其他DNA拓扑异构酶的范例，DNA促旋酶受到了广泛关注。在本综述中，我们通过探讨该酶研究的广泛方面，总结了有关DNA促旋酶的现有知识。
