Liu Jin-man, Yang Ke-qian
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
Wei Sheng Wu Xue Bao. 2006 Feb;46(1):33-7.
In Streptomyces coelicolor A3(2), SCO5433 encodes a TPR domain containing protein designated TcrA (TPR containing regulator A). TcrA is similar in amino acid sequence to AfsR, a well-characterized global regulatory protein in S. coelicolor A3(2). Disruption of tcrA enhanced the production of spore pigment on MM containing glucose or mannitol and also resulted in more diffusible pigment production on MM containing mannitol or MS agar medium, but no significant effects on morphological differentiation were observed. Complementation of tcrA mutation restored the phenotype of tcrA mutant to that of the wild-type strain. These results suggest that tcrA is involved in the regulation of secondary metabolism under defined conditions. In S. coelicolor A3(2), the AfsK/AfsR system positively regulates the production of secondary metabolites while the results of our work suggest that there might be a TcrA-dependent pathway that negatively regulates secondary metabolism.
在天蓝色链霉菌A3(2)中，SCO5433编码一种含有TPR结构域的蛋白质，命名为TcrA（含TPR的调节因子A）。TcrA的氨基酸序列与AfsR相似，AfsR是天蓝色链霉菌A3(2)中一个已被充分研究的全局调节蛋白。tcrA的缺失增强了在含有葡萄糖或甘露醇的MM培养基上孢子色素的产生，并且在含有甘露醇的MM培养基或MS琼脂培养基上也导致了更多可扩散色素的产生，但未观察到对形态分化有显著影响。tcrA突变的互补将tcrA突变体的表型恢复到野生型菌株的表型。这些结果表明，tcrA在特定条件下参与次生代谢的调控。在天蓝色链霉菌A3(2)中，AfsK/AfsR系统正向调节次生代谢产物的产生，而我们的研究结果表明，可能存在一条依赖TcrA的途径负向调节次生代谢。
