Pawlik Krzysztof, Kotowska Magdalena, Kolesiński Piotr
Department of Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
J Mol Microbiol Biotechnol. 2010;19(3):147-51. doi: 10.1159/000321501. Epub 2010 Oct 6.
Streptomyces coelicolor A3(2) is an extensively studied model organism for the genetic studies of Streptomycetes - a genus known for the production of a vast number of bioactive compounds and complex regulatory networks controlling morphological differentiation and secondary metabolites production. We present the discovery of a presumptive product of the Cpk polyketide synthase. We have found that on the rich medium without glucose S. coelicolor A3(2) produces a yellow compound secreted into the medium. We have proved by complementation that production of the observed yellow pigment is dependent on cpk gene cluster previously described as cryptic type I polyketide synthase cluster. The pigment production depends on the medium composition, does not occur in the presence of glucose, and requires high density of spore suspension used for inoculation.
天蓝色链霉菌A3(2)是一种经过广泛研究的模式生物，用于链霉菌的遗传学研究。链霉菌属以产生大量生物活性化合物以及控制形态分化和次级代谢产物产生的复杂调控网络而闻名。我们展示了一种假定的Cpk聚酮合酶产物的发现。我们发现，在不含葡萄糖的丰富培养基上，天蓝色链霉菌A3(2)会产生一种分泌到培养基中的黄色化合物。我们通过互补实验证明，所观察到的黄色色素的产生依赖于先前被描述为隐秘I型聚酮合酶基因簇的cpk基因簇。色素的产生取决于培养基组成，在有葡萄糖存在时不会发生，并且需要用于接种的孢子悬液的高密度。
