Cao Sheng-bo, Sun Min-xuan, Liu Xue-qin, Liu Xin-wen, Chen Huan-chun
State Key Laboratory of Agricultural Microbiology, Hua Zhong Agricultural University, Wuhan 430070, China.
Wei Sheng Wu Xue Bao. 2006 Feb;46(1):158-61.
Porcine circovirus type 2(PCV2) recently emerged as an important infectious pathogen for pigs in the world. Unfortunately, there is no efficient method to deal with PCV2 infection until now. In this study, chimeric porcine circovirus molecular clone (pSK2PCV1-2) was constructed by cloning capsid gene of PCV2 into the backbone of PCV1. PK-15 cells was transfected with pSK2PCV1-2 and then cultivated in plate for five passages. mRNA of PCV1 ORF1 and PCV2 ORF2 were detected in the fifth passage, but mRNA of PCV1 ORF2 and PCV2 ORF1 were not detected in the cells. On the other hand, capsid protein of PCV2 was also detected in the nucleolus of transfected cells by IIF. This study indicated that pSK2PCV1-2 could form infectious virus in transfected cells. It will provide base for further study on biological characteristic of chimeric porcine circovirus.
猪圆环病毒2型（PCV2）最近成为全球猪的一种重要传染性病原体。不幸的是，迄今为止尚无有效的方法来应对PCV2感染。在本研究中，通过将PCV2的衣壳基因克隆到PCV1的骨架中构建了嵌合猪圆环病毒分子克隆（pSK2PCV1-2）。用pSK2PCV1-2转染PK-15细胞，然后在平板中培养五代。在第五代中检测到PCV1 ORF1和PCV2 ORF2的mRNA，但在细胞中未检测到PCV1 ORF2和PCV2 ORF1的mRNA。另一方面，通过间接免疫荧光法（IIF）在转染细胞的核仁中也检测到了PCV2的衣壳蛋白。本研究表明pSK2PCV1-2可在转染细胞中形成感染性病毒。这将为进一步研究嵌合猪圆环病毒的生物学特性提供基础。
