Stafford S A, Bowery N G, Tang K, Coote J H
Division of Neuroscience, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Neuroscience. 2006 Jul 7;140(3):1031-40. doi: 10.1016/j.neuroscience.2006.02.039. Epub 2006 Mar 31.
In urethane-anesthetized male rats, a branch of the hypogastric nerve was shown, anatomically and electrophysiologically, to supply the vas deferens. Recordings from this nerve revealed a low level of tonic activity, which was predominantly efferent motor activity. Administration of p-chloroamphetamine i.v., elicited a rhythmic burst of neuronal activity, coherent with rhythmic pressure increases in the vas deferens and contractions of the bulbospongiosus muscles, which together comprise ejaculation. This response to p-chloroamphetamine was still present after complete transection of the spinal cord at T8-T9. These data indicate that p-chloroamphetamine is capable of activating the spinal neuronal circuits that generate the pattern of autonomic and somatic responses similar to those of sexual climax. Furthermore based on the best documented action of p-chloroamphetamine, the results suggest that the excitability of the pattern generator is regulated by serotonergic, dopaminergic or noradrenergic receptors in the lumbosacral spinal cord. We conclude this animal model will enable robust studies of the pharmacology and physiology of central neural mechanisms involved in ejaculation and sexual climax.
在氨基甲酸乙酯麻醉的雄性大鼠中，通过解剖学和电生理学方法证实，腹下神经的一个分支为输精管提供支配。对该神经的记录显示存在低水平的紧张性活动，主要是传出性运动活动。静脉注射对氯苯丙胺可引发神经元活动的节律性爆发，这与输精管内的节律性压力升高以及球海绵体肌的收缩相一致，这些共同构成射精过程。在T8 - T9水平完全横断脊髓后，对氯苯丙胺的这种反应仍然存在。这些数据表明，对氯苯丙胺能够激活脊髓神经元回路，从而产生类似于性高潮的自主和躯体反应模式。此外，基于对氯苯丙胺最充分记录的作用，结果表明模式发生器的兴奋性受腰骶脊髓中5-羟色胺能、多巴胺能或去甲肾上腺素能受体调节。我们得出结论，该动物模型将有助于对射精和性高潮所涉及的中枢神经机制的药理学和生理学进行深入研究。
