Petric M, Mayur K, Vonderfecht S, Eiden J J
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
J Gen Virol. 1991 Nov;72 ( Pt 11):2801-4. doi: 10.1099/0022-1317-72-11-2801.
Group B rotaviruses (GBRs) were recognized recently as causative agents of gastroenteritis. Investigations into the relatedness of various heterologous GBR strains have been hindered by the difficulty of growing these viruses in cell culture. Viral RNA extracted from experimentally infected rats used to prepare cDNA clones. From these, the nucleotide sequences of genes 9 and 11 of the IDIR strain of GBR were determined and compared with the corresponding sequences of the human ADRV strain of GBR. IDIR gene 11 is 643 bp in length with a single open reading frame (ORF) encoding 174 amino acids; IDIR gene 9 is 804 bp in length with a single ORF encoding 246 amino acids. Comparison of the IDIR sequences with those of ADRV showed that nucleotide sequence similarity was 60.6% and 71.9% for genes 9 and 11, respectively. The deduced amino acid sequence similarity was 51.2% for the gene 9 and 66.5% for the gene 11 product. This sequence diversity indicates that GBRs are more distantly related than strains of group A rotavirus.
B组轮状病毒（GBRs）最近被确认为肠胃炎的病原体。由于这些病毒在细胞培养中难以生长，对各种异源GBR毒株相关性的研究受到了阻碍。从实验感染的大鼠中提取病毒RNA用于制备cDNA克隆。由此确定了GBR IDIR毒株基因9和11的核苷酸序列，并与GBR人ADRV毒株的相应序列进行了比较。IDIR基因11长度为643 bp，有一个单一的开放阅读框（ORF），编码174个氨基酸；IDIR基因9长度为804 bp，有一个单一的ORF，编码246个氨基酸。将IDIR序列与ADRV序列进行比较，结果显示基因9和11的核苷酸序列相似性分别为60.6%和71.9%。推导的氨基酸序列相似性，基因9为51.2%，基因11产物为66.5%。这种序列多样性表明，GBRs之间的亲缘关系比A组轮状病毒毒株更为疏远。
