Chang K O, Parwani A V, Smith D, Saif L J
Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA.
J Clin Microbiol. 1997 Aug;35(8):2107-10. doi: 10.1128/jcm.35.8.2107-2110.1997.
Groups A, B, and C rotaviruses have been identified in cattle. Group B rotaviruses are associated with sporadic cases of diarrhea in calves and adult cows. From diagnostic submissions to our laboratory, 90 fecal samples from cases of calf diarrhea, 81 fecal samples from cases of adult cow diarrhea (winter dysentery), and 20 fecal samples from case control normal adult cows were tested for group B rotaviruses by polyacrylamide gel electrophoresis (PAGE), and reverse transcription (RT)-PCR (targeting 279 bp of the VP7 gene). In addition, 53 fecal samples from diarrheic adult cows were tested for group B rotaviruses by immune electron microscopy (IEM). By RT-PCR, five samples from calves were group B rotavirus positive (5.6%). Fifteen samples from adult cows with diarrhea were group B rotavirus positive (18.5%), and none of the control fecal samples from normal cows were positive for group B rotaviruses. By PAGE, one calf sample (RT-PCR positive) was group B rotavirus positive (short electropherotype), but none of the adult cow samples were positive for group B rotaviruses. By IEM, 5 (9.4%) of the 53 fecal samples from diarrheic adult cows were group B positive (all were also RT-PCR positive). The VP7 genes of three strains (WD653 from an adult cow and the ATI and Mebus calf strains) were sequenced. The VP7 genes from the three bovine strains showed high (over 90%) nucleotide and deduced amino acid homologies, but lower homologies (48 to 61%) were seen between these genes and the genes from rodent (IDIR) and human (ADRV) group B rotaviruses. Although there were some differences of degree, all inoculated gnotobiotic calves (n = 6) showed abnormal feces between 1 and 3 days after inoculation with each of three strains of group B bovine rotaviruses, and group B rotaviruse, were detected in the feces for up to 2 weeks by RT-PCR but for shorter periods by PAGE or IEM.
已在牛中鉴定出A、B和C组轮状病毒。B组轮状病毒与犊牛和成年母牛的散发性腹泻病例有关。从提交到我们实验室进行诊断的样本中，采用聚丙烯酰胺凝胶电泳（PAGE）和逆转录（RT）-PCR（靶向VP7基因的279 bp）对90份犊牛腹泻病例的粪便样本、81份成年母牛腹泻（冬季痢疾）病例的粪便样本以及20份病例对照正常成年母牛的粪便样本进行了B组轮状病毒检测。此外，采用免疫电镜（IEM）对53份成年腹泻母牛的粪便样本进行了B组轮状病毒检测。通过RT-PCR，5份犊牛样本为B组轮状病毒阳性（5.6%）。15份成年腹泻母牛样本为B组轮状病毒阳性（18.5%），正常母牛的对照粪便样本均未检测出B组轮状病毒阳性。通过PAGE，1份犊牛样本（RT-PCR阳性）为B组轮状病毒阳性（短电泳型），但成年母牛样本均未检测出B组轮状病毒阳性。通过IEM，53份成年腹泻母牛粪便样本中有5份（9.4%）为B组阳性（均也为RT-PCR阳性）。对三株病毒（成年母牛的WD653以及ATI和Mebus犊牛株）的VP7基因进行了测序。这三株牛病毒的VP7基因显示出较高的（超过90%）核苷酸和推导氨基酸同源性，但这些基因与啮齿动物（IDIR）和人类（ADRV）B组轮状病毒的基因之间的同源性较低（48%至61%）。尽管在程度上存在一些差异，但所有接种的无菌犊牛（n = 6）在接种三株B组牛轮状病毒中的每一株后1至3天均出现粪便异常，通过RT-PCR在粪便中检测到B组轮状病毒长达2周，但通过PAGE或IEM检测到的时间较短。
