Yang Jiang-Cun, Li Feng-Qin, Li Mang-Hui, Ren Jian-Kang
Department of Transfusion Blood, Shanxi Provincial People's Hospital, Xi'an 710068, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Feb;14(1):162-4.
The study was to explore the change of coagulation factor VIII and IX activities in the platelet suspension collected by platelet apheresis during storage at 22 degrees C. 18 samples of platelet concentrates were collected by the cs-3000 plus and stored at 22 degrees C and then FVIII: C, FIX: C activities were detected at 0, 12, 24, 48, 72, 96, 120 hours respectively by SYSMEX CA-1500. The results showed that FVIII: C activity was (100.51 + 44.02)% at 0 hour, and then decreased dramatically to 10% - 40% of primary level from 12 to 120 hours, while FIX: C activity was (120.93 +/- 20.50)% at 0 hour and decreased to 10% - 35% of primary level from 24 to 120 hours. In conclusion, FVIII and FIX in the platelet concentrates stored at 22 degrees C could keep their biological activities at physiologically high levels.
本研究旨在探讨采用血细胞分离机采集的血小板悬液在22℃储存期间凝血因子Ⅷ和Ⅸ活性的变化。通过cs - 3000 plus采集18份血小板浓缩液样本，并在22℃储存，然后分别于0、12、24、48、72、96、120小时采用SYSMEX CA - 1500检测FVIII:C、FIX:C活性。结果显示，FVIII:C活性在0小时为(100.51 + 44.02)%，从12小时至120小时急剧下降至初始水平的10% - 40%，而FIX:C活性在0小时为(120.93 +/- 20.50)%，从24小时至120小时下降至初始水平的10% - 35%。综上所述，在22℃储存的血小板浓缩液中的FVIII和FIX可在生理高水平维持其生物活性。
