一种类酶合成聚合物实现了10倍的催化加速。
Catalytic accelerations of 10-fold by an enzyme-like synthetic polymer.
作者信息
Kiefer H C, Congdon W I, Scarpa I S, Klotz I M
机构信息
Biochemistry Division, Department of Chemistry, Northwestern University, Evanston, Illinois 60201.
出版信息
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2155-9. doi: 10.1073/pnas.69.8.2155.
Abstract
A synthetic polymer, polyethyleneimine containing dodecyl (C(12)H(25))-chains for binding sites and imidazole moieties for functional catalytic groups, catalyzes the hydrolysis of phenolic sulfate esters by a two-step mechanism resembling that of the corresponding natural enzyme. Quantitative rate studies give kinetic parameters that show that the polymer (synzyme) accelerates the rate 10(12)-fold compared to unbound imidazole, and 10(2)-fold compared to a type IIA aryl sulfatase enzyme.
摘要
一种合成聚合物，即含有十二烷基（C(12)H(25)）链作为结合位点和咪唑部分作为功能性催化基团的聚乙烯亚胺，通过一种类似于相应天然酶的两步机制催化酚硫酸酯的水解。定量速率研究给出的动力学参数表明，与未结合的咪唑相比，该聚合物（合成酶）将反应速率提高了10^12倍，与IIA型芳基硫酸酯酶相比提高了10^2倍。
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本文引用的文献
1
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2
Studies in detoxication. XXXXIII. A study of the arylsulphatase activity of takadiastase towards some phenolic ethereal sulphates.解毒作用的研究。第四十三部分。对淀粉酶对某些酚醚硫酸盐的芳基硫酸酯酶活性的研究。
Biochem J. 1952 May;51(2):202-8. doi: 10.1042/bj0510202.
3
MECHANISM OF ACTION OF PROTEOLYTIC ENZYMES.蛋白水解酶的作用机制
Annu Rev Biochem. 1965;34:49-76. doi: 10.1146/annurev.bi.34.070165.000405.
4
The kinetics of the alpha-chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate.α-胰凝乳蛋白酶催化乙酸对硝基苯酯水解的动力学
Biochemistry. 1962 Nov;1:1097-106. doi: 10.1021/bi00912a021.
5
The ultramicro determination of inorganic sulphte.无机硫酸盐的超微量测定
Biochem J. 1960 Jun;75(3):435-40. doi: 10.1042/bj0750435.
6
The sulphatase of ox liver. XII. The effect of tyrosine and histidine reagents on the activity of sulphatase A.
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