Bedbrook J R, Bogorad L
The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309-13. doi: 10.1073/pnas.73.12.4309.
The closed-circular DNA molecules of 85 x 10(6) daltons from Zea mays chloroplasts were isolated, digested with the restriction endonucleases Sal I, Bam I, and EcoRI, and the resulting fragments sized by agarose gel electrophoresis. A map of maize chloroplast DNA showing the relative location of all the Sal I recognition sequences and many of the Bam I and EcoRI recognition sites was determined. A DNA sequence representing approximately 15% of the Zea mays chloroplast genome is repeated. The two copies of this sequence are in an inverted orientation with respect to one another and are separated by a nonhomologous sequence representing approximately 10% of the genome length. The inverted repeats contain the genes for chloroplast ribosomal RNAs.
从玉米叶绿体中分离出分子量为85×10⁶道尔顿的闭环DNA分子，用限制性内切酶Sal I、Bam I和EcoRI进行消化，然后通过琼脂糖凝胶电泳对所得片段进行大小测定。确定了玉米叶绿体DNA图谱，该图谱显示了所有Sal I识别序列以及许多Bam I和EcoRI识别位点的相对位置。代表玉米叶绿体基因组约15%的一段DNA序列是重复的。该序列的两个拷贝彼此呈反向排列，并被一段代表基因组长度约10%的非同源序列隔开。反向重复序列包含叶绿体核糖体RNA的基因。
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