Whitfeld P R, Herrmann R G, Bottomley W
Nucleic Acids Res. 1978 Jun;5(6):1741-51. doi: 10.1093/nar/5.6.1741.
Spinach chloroplast ribosomal RNAs have been hybridized to restriction endonuclease fragments of spinach chloroplast DNA. All three RNA species (23S, 16S and 5S) hybridized to a single large fragment when the DNA was digested with either Sall or Pstl. Hybridization of 23S RNA to fragments produced by Smal yielded two radioactive bands which corresponded to the bi-molar 2.5 X 10(6) and 1.15 X 10(6) Mr fragments. 16S RNA also hybridized to two, bi-molar Smal fragments (3.4 X 10(6) and 2.5 X 10(6) Mr) and 5S RNA hybridized to the 1.15 X 10(6) Mr bi-molar Smal fragment. The 23S RNA and 16S RNA cistrons were each also shown to contain a single EcoRI site. From the data it was possible to conclude that the ribosomal RNA genes are located on the inverted repeat region of the spinach chloroplast DNA restriction map [1,2], that the sequence of the cistrons is 16S - 23S - 5S and that the size of the spacer between the 16S and 23S RNA cistrons is approximately 0.90 X 10(6) Mr.
菠菜叶绿体核糖体RNA已与菠菜叶绿体DNA的限制性内切酶片段进行杂交。当用SalI或PstI消化DNA时，所有三种RNA种类（23S、16S和5S）都与一个单一的大片段杂交。23S RNA与SmaI产生的片段杂交产生了两条放射性条带，分别对应于双分子的2.5×10⁶和1.15×10⁶道尔顿的片段。16S RNA也与两个双分子的SmaI片段（3.4×10⁶和2.5×10⁶道尔顿）杂交，5S RNA与1.15×10⁶道尔顿的双分子SmaI片段杂交。23S RNA和16S RNA顺反子各自还显示含有一个单一的EcoRI位点。从这些数据可以得出结论，核糖体RNA基因位于菠菜叶绿体DNA限制性图谱的反向重复区域[1,2]，顺反子的序列为16S - 23S - 5S，并且16S和23S RNA顺反子之间间隔区的大小约为0.90×10⁶道尔顿。
