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荧光法测定二肽基羧肽酶（血管紧张素I转换酶）。

Fluorimetric determination of dipeptidyl carboxypeptidase. (angiotensin-I-converting enzyme).

作者信息

Depierre D, Roth M

出版信息

Enzyme. 1975;19(2):65-70. doi: 10.1159/000458975.

DOI:10.1159/000458975
PMID:165929
Abstract

A fluorimetric assay of dipeptidyl carboxypeptidase is described. It involves incubation at 37 degrees C with the substrate, Z-Phe-His-Leu, and reaction of the dipeptide His-Leu which is released upon enzymatic hydrolysis, with o-phthalaldehyde to yield a fluorescent compound. The method is simple, precise and sensitive. The assay in serum is conveniently performed on 20 mul samples. Normal values in human serum range from 0.04 to 0.22 U/l.

摘要

本文描述了一种二肽基羧肽酶的荧光测定法。该方法包括在37℃下与底物Z-苯丙氨酸-组氨酸-亮氨酸孵育，酶促水解释放出的二肽组氨酸-亮氨酸与邻苯二甲醛反应生成一种荧光化合物。该方法简单、精确且灵敏。血清检测可方便地在20微升样本上进行。人血清中的正常值范围为0.04至0.22 U/l。

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Fluorimetric determination of dipeptidyl carboxypeptidase. (angiotensin-I-converting enzyme).荧光法测定二肽基羧肽酶（血管紧张素I转换酶）。
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A fluorimetric assay for angiotensin-I converting enzyme in human serum.
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