Rietz B, Isensee H, Strobach H, Makdessi S, Jacob R
Physiologisches Institut II, Universität Tübingen/FRG.
Mol Cell Biochem. 1993 Feb 17;119(1-2):143-50. doi: 10.1007/BF00926865.
The susceptibility to ventricular arrhythmias under the conditions of cardiac ischemia and reperfusion was investigated in the Langendorff heart preparation of rats fed for eight weeks a standard chow enriched with 2% of pulverized wild garlic leaves. The isolated hearts were perfused with a modified Krebs-Henseleit solution. The incidence of ventricular fibrillation (VF) during 20 min occlusion of the descending branch of the left coronary artery (LAD) was significantly reduced in the wild garlic group as compared to untreated controls (20% vs 88%). The same holds for the size of the ischemic zone (33.6% vs 40.9% of heart weight). In the reperfusion experiments (5 min after 10 min ischemia), ventricular tachycardia (VT) occurred in 70% of the wild garlic group vs 100% in untreated controls and VF in 50% vs 90%. The time until occurrence of extrasystoles, VT or VR was prolonged. No significant alterations in cardiac fatty acid composition could be observed. Although the prostacyclin production was slightly increased in hearts of the wild garlic group, inhibition of cyclooxygenase by acetylsalicylic acid (ASA; aspirin) could not completely prevent the cardioprotective effects suggesting that the prostaglandin system does not play a decisive role in the cardioprotective action of wild garlic. Furthermore, a moderate angiotensin converting enzyme (ACE) inhibiting action of wild garlic was found in vitro as well as in vivo that could contribute to the cardioprotective and blood pressure lowering action of wild garlic. Whether a free radical scavenging activity of wild garlic is involved in its cardioprotective effects remains to be established.
在Langendorff大鼠心脏制备模型中，研究了连续8周喂食富含2%大蒜叶粉标准饲料的大鼠在心脏缺血和再灌注条件下对室性心律失常的易感性。分离的心脏用改良的Krebs-Henseleit溶液灌注。与未处理的对照组相比，大蒜组在左冠状动脉降支（LAD）闭塞20分钟期间室颤（VF）的发生率显著降低（20%对88%）。缺血区大小也是如此（占心脏重量的33.6%对40.9%）。在再灌注实验中（缺血10分钟后5分钟），大蒜组70%出现室性心动过速（VT），未处理对照组为100%；大蒜组50%出现室颤，未处理对照组为90%。早搏、VT或VF出现的时间延长。未观察到心脏脂肪酸组成有明显变化。虽然大蒜组心脏中前列环素的产生略有增加，但乙酰水杨酸（ASA；阿司匹林）抑制环氧化酶并不能完全阻止其心脏保护作用，这表明前列腺素系统在大蒜的心脏保护作用中不起决定性作用。此外，在体外和体内均发现大蒜具有适度的血管紧张素转换酶（ACE）抑制作用，这可能有助于大蒜的心脏保护和降压作用。大蒜的心脏保护作用是否涉及其自由基清除活性尚待确定。
