大肠杆菌中硫酸盐还原的正调控。大肠杆菌K12多效性无半胱氨酸突变体的性质。
Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12.
作者信息
Jones-Mortimer M C
出版信息
Biochem J. 1968 Dec;110(3):597-602. doi: 10.1042/bj1100597.
Abstract
- The function of the wild-type alleles of the pleiotropic mutants cysB and cysE of Escherichia coli was investigated. 2. The wild-type allele cysB(+) is dominant to the mutant allele cysB in stable and transient heterozygotes. 3. The wild-type allele cysE(+) is dominant to the mutant allele cysE, as predicted. 4. Sulphur-starved cultures of cysB or cysE strains contain less than 0.2nmole of free cysteine/mg. dry wt. 5. Complementation in vitro is not observed between extracts of cysB mutants and mutants lacking sulphite reductase only. 6. A scheme, involving positive control of the enzymes of sulphate activation and reduction, is suggested to account for the control of cysteine biosynthesis.
摘要
- 对大肠杆菌多效突变体cysB和cysE的野生型等位基因的功能进行了研究。2. 在稳定和瞬时杂合子中，野生型等位基因cysB(+)对突变等位基因cysB呈显性。3. 如预期的那样，野生型等位基因cysE(+)对突变等位基因cysE呈显性。4. cysB或cysE菌株的硫饥饿培养物中，每毫克干重所含的游离半胱氨酸少于0.2纳摩尔。5. 在cysB突变体提取物与仅缺乏亚硫酸盐还原酶的突变体之间未观察到体外互补作用。6. 提出了一个涉及硫酸盐活化和还原酶正调控的方案，以解释半胱氨酸生物合成的调控。
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