Institut für Genetik der Universität Bonn, Kirschallee 1, D-5300, Bonn 1, Federal Republic of Germany.
Plant Cell Rep. 1987 Feb;6(1):50-4. doi: 10.1007/BF00269738.
Polypeptide pattern alterations during somatic embryogenesis were investigated using callus cultures of two Pisum sativum genotypes. Both genotypes show the formation of two different callus lines from the same explant after six to eight weeks in culture: a nodular yellowish callus line, which forms somatic embryoids in suspension cultures (e(+)) and a white compact callus line with no regenerative capacity (e(-)). The cytosol proteins of the two different callus lines were separated in a semi-preparative two-dimensional system and the polypeptide patterns were compared. Two protein bands were found (P1: Mr=45000 D, pI=7.0-7.1; P2: Mr=7000 D, pI=<4.5), which were characteristic of the putatively embryogenic (e(+)) callus line in all tissues investigated (two genotypes × two explant sources). These proteins found in nodular (e(+)) pea cultures are very similar to two proteins found in Daucus carota suspension cultures preceding the formation of somatic embryos.
利用两个豌豆品种的愈伤组织培养物研究体细胞胚胎发生过程中的多肽模式变化。在培养 6-8 周后，两种基因型都从相同的外植体中形成两种不同的愈伤组织系：一种是结节状黄色愈伤组织系，它在悬浮培养中形成体细胞胚状体（e(+)），另一种是没有再生能力的白色致密愈伤组织系（e(-)）。两种不同愈伤组织系的胞质蛋白在半制备二维系统中分离，并比较了多肽图谱。发现了两条蛋白带（P1：Mr=45000 D，pI=7.0-7.1；P2：Mr=7000 D，pI<4.5），它们是所有研究组织（两种基因型×两种外植体来源）中推测的胚胎发生（e(+)）愈伤组织系的特征。在结节状（e(+)）豌豆培养物中发现的这些蛋白质与在体细胞胚胎形成前的胡萝卜悬浮培养物中发现的两种蛋白质非常相似。
