马铃薯纺锤块茎类病毒复制中的 RNA 中间体。
RNA intermediates in potato spindle tuber viroid replication.
机构信息
Plant Virology Laboratory, Plant Protection Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
出版信息
Proc Natl Acad Sci U S A. 1982 Jan;79(1):113-7. doi: 10.1073/pnas.79.1.113.
Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA-RNA hybridization techniques. These replicative intermediates contain monomeric circular or linear PSTV strands complexed with a multimeric complementary RNA strand. Synchronous synthesis of single-stranded PSTV is accompained by a simultaneous marked increase in double-stranded PSTV RNA; thus, in vivo precursors of the characterized double-stranded PSTV RNAs seem to be involved in PSTV replication. A "rolling circle" model for viroid replication on a circular PSTV template can accommodate the double-stranded PSTV RNA species characterized.
已从感染马铃薯纺锤块茎类病毒 (PSTV)的番茄组织中分离到 PSTV 复制的两种双链 RNA 中间体，并通过聚丙烯酰胺凝胶电泳和 DNA-RNA 杂交技术进行了表征。这些复制中间体包含与多聚互补 RNA 链复合的单体环状或线状 PSTV 链。单链 PSTV 的同步合成伴随着双链 PSTV RNA 的同时显著增加；因此，所鉴定的双链 PSTV RNA 的体内前体似乎参与了 PSTV 的复制。一种基于圆形 PSTV 模板的类病毒复制的“滚环”模型可以容纳所鉴定的双链 PSTV RNA 物种。
Zotero 插件