Plant Virology Laboratory, Plant Protection Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705.
Proc Natl Acad Sci U S A. 1982 Jan;79(1):113-7. doi: 10.1073/pnas.79.1.113.
Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA-RNA hybridization techniques. These replicative intermediates contain monomeric circular or linear PSTV strands complexed with a multimeric complementary RNA strand. Synchronous synthesis of single-stranded PSTV is accompained by a simultaneous marked increase in double-stranded PSTV RNA; thus, in vivo precursors of the characterized double-stranded PSTV RNAs seem to be involved in PSTV replication. A "rolling circle" model for viroid replication on a circular PSTV template can accommodate the double-stranded PSTV RNA species characterized.
已从感染马铃薯纺锤块茎类病毒 (PSTV)的番茄组织中分离到 PSTV 复制的两种双链 RNA 中间体，并通过聚丙烯酰胺凝胶电泳和 DNA-RNA 杂交技术进行了表征。这些复制中间体包含与多聚互补 RNA 链复合的单体环状或线状 PSTV 链。单链 PSTV 的同步合成伴随着双链 PSTV RNA 的同时显著增加；因此，所鉴定的双链 PSTV RNA 的体内前体似乎参与了 PSTV 的复制。一种基于圆形 PSTV 模板的类病毒复制的“滚环”模型可以容纳所鉴定的双链 PSTV RNA 物种。
