蓝藻酶与菠菜核 DNA 编码的质体特异性同工酶的免疫学相似性。
Immunological similarity between a cyanobacterial enzyme and a nuclear DNA-encoded plastid-specific isozyme from spinach.
机构信息
Department of Genetics, University of California, Davis, California 95616.
出版信息
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5953-5. doi: 10.1073/pnas.79.19.5953.
The immunochemical properties of the plastid and cytosolic isozymes of phosphoglucose isomerase (glucosephosphate isomerase; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) in spinach (Spinacia oleracea) and the single phosphoglucose isomerase enzyme from the cyanobacterium Synechococcus sp. were compared by an application of the enzyme-linked immunosorbent assay. Utilizing antibodies made in rabbits against subunits of purified plastid and cytosolic phosphoglucose isomerase isozymes from spinach, we demonstrate that the plastid isozyme is immunochemically more similar to the cyanobacterial enzyme than to the spinach cytosolic counterpart. The antiserum to plastid phosphoglucose isomerase crossreacted strongly with plastid phosphoglucose isomerases from other flowering plants. The antiserum to cytosolic phosphoglucose isomerase crossreacted with other plant cytosolic phosphoglucose isomerase isozymes. The results are consistent with the hypothesis [Weeden, N. F. (1981) J. Mol. Evol. 17, 133-139] that the nuclear gene specifying plastid phosphoglucose isomerase was derived from a prokaryote after the incorporation of a plastid-like symbiont into the ancestral plant cell.
采用酶联免疫吸附测定法比较了菠菜（Spinacia oleracea）质体和胞质同工型磷酸葡萄糖异构酶（磷酸葡萄糖异构酶；D-葡萄糖-6-磷酸酮醇异构酶，EC 5.3.1.9）的免疫化学性质和来自蓝细菌 Synechococcus sp. 的单一磷酸葡萄糖异构酶酶。利用针对从菠菜纯化的质体和胞质磷酸葡萄糖异构酶同工型的亚基制成的兔抗体制备物，我们证明质体同工型在免疫化学上与蓝细菌酶比与菠菜胞质同工型更相似。抗质体磷酸葡萄糖异构酶的抗血清与来自其他开花植物的质体磷酸葡萄糖异构酶强烈交叉反应。抗胞质磷酸葡萄糖异构酶的抗血清与其他植物胞质磷酸葡萄糖异构酶同工型发生交叉反应。这些结果与假设一致[Weeden，NF（1981）J. Mol. Evol. 17, 133-139]，即指定质体磷酸葡萄糖异构酶的核基因是在将类似质体的共生体纳入祖细胞后，从原核生物衍生而来的。
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