Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK.
Plant Mol Biol. 1985 Nov;4(6):333-45. doi: 10.1007/BF02418255.
The nucleotide sequences of the chloroplast genes for the alpha, beta and epsilon subunits of wheat chloroplast ATP synthase have been determined. Open reading frames of 1512 bp, 1494 bp and 411 bp are deduced to code for polypeptides of molecular weights 55201, 53796 and 15200, identified as the alpha, beta and epsilon subunits respectively by homology with the subunits from other sources and by amino acid sequencing of the epsilon subunit. The genes for the beta and epsilon subunits overlap by 4 bp. The gene for methionine tRNA is located 118 bp downstream from the epsilon subunit gene. Comparisons of the deduced amino acid sequences of the alpha and beta subunits with those from other species suggest regions of the proteins involved in adenine nucleotide binding.
已测定了小麦叶绿体 ATP 合酶的α、β和ε亚基的叶绿体基因的核苷酸序列。推断出的开放阅读框分别为 1512bp、1494bp 和 411bp，编码分子量为 55201、53796 和 15200 的多肽，通过与其他来源的亚基的同源性以及通过 ε 亚基的氨基酸测序将其分别鉴定为 α、β 和 ε 亚基。β和ε亚基的基因重叠 4bp。甲硫氨酸 tRNA 的基因位于 ε 亚基基因下游 118bp 处。α和β亚基的推导氨基酸序列与其他物种的比较表明，这些蛋白质涉及腺嘌呤核苷酸结合的区域。
