叶绿体 ATP 合酶：从结构到工程。
Chloroplast ATP synthase: From structure to engineering.
机构信息
Plant Molecular Biology, Faculty of Biology, Ludwig-Maximilians-University Munich, D-82152 Planegg-Martinsried, Germany.
出版信息
Plant Cell. 2024 Oct 3;36(10):3974-3996. doi: 10.1093/plcell/koae081.
Abstract
F-type ATP synthases are extensively researched protein complexes because of their widespread and central role in energy metabolism. Progress in structural biology, proteomics, and molecular biology has also greatly advanced our understanding of the catalytic mechanism, post-translational modifications, and biogenesis of chloroplast ATP synthases. Given their critical role in light-driven ATP generation, tailoring the activity of chloroplast ATP synthases and modeling approaches can be applied to modulate photosynthesis. In the future, advances in genetic manipulation and protein design tools will significantly expand the scope for testing new strategies in engineering light-driven nanomotors.
摘要
F 型 ATP 合酶是广泛研究的蛋白质复合物，因为它们在能量代谢中具有广泛而核心的作用。结构生物学、蛋白质组学和分子生物学的进展也极大地促进了我们对叶绿体 ATP 合酶的催化机制、翻译后修饰和生物发生的理解。鉴于它们在光驱动 ATP 生成中的关键作用，可以调整叶绿体 ATP 合酶的活性和建模方法来调节光合作用。在未来，遗传操作和蛋白质设计工具的进步将大大扩展测试工程光驱动纳米马达新策略的范围。
相似文献
1
Chloroplast ATP synthase: From structure to engineering.叶绿体 ATP 合酶：从结构到工程。
Plant Cell. 2024 Oct 3;36(10):3974-3996. doi: 10.1093/plcell/koae081.
2
PAB is an assembly chaperone that functions downstream of chaperonin 60 in the assembly of chloroplast ATP synthase coupling factor 1.PAB是一种组装伴侣蛋白，在叶绿体ATP合酶偶联因子1的组装过程中，它在伴侣蛋白60的下游发挥作用。
Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):4152-7. doi: 10.1073/pnas.1413392111. Epub 2015 Mar 16.
3
Impact of engineering the ATP synthase rotor ring on photosynthesis in tobacco chloroplasts.工程化 ATP 合酶转子环对烟草叶绿体光合作用的影响。
Plant Physiol. 2023 May 31;192(2):1221-1233. doi: 10.1093/plphys/kiad043.
4
The Arabidopsis Protein CGLD11 Is Required for Chloroplast ATP Synthase Accumulation.拟南芥蛋白 CGLD11 对于叶绿体 ATP 合酶的积累是必需的。
Mol Plant. 2016 Jun 6;9(6):885-99. doi: 10.1016/j.molp.2016.03.002. Epub 2016 Mar 12.
5
Structure and Mechanisms of F-Type ATP Synthases.F 型 ATP 合酶的结构与机制。
Annu Rev Biochem. 2019 Jun 20;88:515-549. doi: 10.1146/annurev-biochem-013118-110903. Epub 2019 Mar 22.
6
The chloroplast ATP synthase features the characteristic redox regulation machinery.叶绿体 ATP 合酶具有典型的氧化还原调控机制。
Antioxid Redox Signal. 2013 Nov 20;19(15):1846-54. doi: 10.1089/ars.2012.5044. Epub 2013 Jan 3.
7
Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β.鉴定叶绿体 ATP 合酶β亚基 N 端磷酸化缺陷的突变体。
Plant Physiol. 2023 Mar 17;191(3):1818-1835. doi: 10.1093/plphys/kiad013.
8
Regulation of proton flow and ATP synthesis in chloroplasts.叶绿体中质子流与ATP合成的调控
J Bioenerg Biomembr. 2000 Oct;32(5):501-6. doi: 10.1023/a:1005669008974.
9
Two specific domains of the γ subunit of chloroplast FF provide redox regulation of the ATP synthesis through conformational changes.叶绿体 FF 的γ亚基的两个特定结构域通过构象变化提供对 ATP 合成的氧化还原调节。
Proc Natl Acad Sci U S A. 2023 Feb 7;120(6):e2218187120. doi: 10.1073/pnas.2218187120. Epub 2023 Jan 30.
10
A Nucleus-Encoded Chloroplast Protein YL1 Is Involved in Chloroplast Development and Efficient Biogenesis of Chloroplast ATP Synthase in Rice.一个核编码的叶绿体蛋白 YL1 参与叶绿体的发育和叶绿体 ATP 合酶在水稻中的高效生物发生。
Sci Rep. 2016 Sep 2;6:32295. doi: 10.1038/srep32295.
引用本文的文献
1
Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants.陆地植物中光合蛋白的组装依赖性翻译反馈调节。
Nat Plants. 2025 Aug 18. doi: 10.1038/s41477-025-02074-x.
2
Functional relocation of the maize chloroplast atpB gene to the nucleus restores photosynthetic competence to a gene-edited non-photosynthetic mutant.玉米叶绿体atpB基因向细胞核的功能重定位恢复了基因编辑的非光合突变体的光合能力。
Plant Biotechnol J. 2025 Sep;23(9):3510-3523. doi: 10.1111/pbi.70175. Epub 2025 Jun 4.
3
Evaluating the influence of phosphorus supply on photosynthetic and agronomic performance of two breeding lines of common bean grown under acidic soil and high temperature stress.评估磷供应对在酸性土壤和高温胁迫条件下种植的两个菜豆育种系光合性能和农艺性能的影响。
PLoS One. 2025 May 28;20(5):e0324863. doi: 10.1371/journal.pone.0324863. eCollection 2025.
4
Integrative Analysis of Transcriptomic and Metabolomic Profiles Uncovers the Mechanism of Color Variation in the Tea Plant Callus.转录组和代谢组谱的综合分析揭示茶树愈伤组织颜色变异的机制
Plants (Basel). 2025 May 13;14(10):1454. doi: 10.3390/plants14101454.
5
Structure, regulation and assembly of the photosynthetic electron transport chain.光合电子传递链的结构、调控与组装
Nat Rev Mol Cell Biol. 2025 May 21. doi: 10.1038/s41580-025-00847-y.
6
Structure of ATP synthase from an early photosynthetic bacterium .来自早期光合细菌的ATP合酶结构
Proc Natl Acad Sci U S A. 2025 Apr;122(13):e2425824122. doi: 10.1073/pnas.2425824122. Epub 2025 Mar 25.
7
ATP Synthase Members of Chloroplasts and Mitochondria in Rubber Trees () Response to Plant Hormones.橡胶树叶绿体和线粒体中的ATP合酶成员对植物激素的响应
Plants (Basel). 2025 Feb 17;14(4):604. doi: 10.3390/plants14040604.
8
Focus on photosynthesis.专注于光合作用。
Plant Cell. 2024 Oct 3;36(10):3895-3896. doi: 10.1093/plcell/koae204.
9
Structure, Regulation, and Significance of Cyanobacterial and Chloroplast Adenosine Triphosphate Synthase in the Adaptability of Oxygenic Photosynthetic Organisms.蓝藻和叶绿体三磷酸腺苷合酶在产氧光合生物适应性中的结构、调控及意义
Microorganisms. 2024 May 6;12(5):940. doi: 10.3390/microorganisms12050940.
本文引用的文献
1
Chloroplast ATP synthase biogenesis requires peripheral stalk subunits AtpF and ATPG and stabilization of atpE mRNA by OPR protein MDE1.叶绿体ATP合酶的生物合成需要外周柄亚基AtpF和ATPG，以及OPR蛋白MDE1对atpE mRNA的稳定作用。
Plant J. 2023 Dec;116(6):1582-1599. doi: 10.1111/tpj.16448. Epub 2023 Oct 12.
2
Optimizing the electron transport chain to sustainably improve photosynthesis.优化电子传递链以可持续地提高光合作用。
Plant Physiol. 2023 Nov 22;193(4):2398-2412. doi: 10.1093/plphys/kiad490.
3
Improving photosynthetic efficiency toward food security: Strategies, advances, and perspectives.提高光合作用效率以保障粮食安全：策略、进展与展望。
Mol Plant. 2023 Oct 2;16(10):1547-1563. doi: 10.1016/j.molp.2023.08.017. Epub 2023 Sep 1.
4
The interplay of post-translational protein modifications in Arabidopsis leaves during photosynthesis induction.在光合作用诱导过程中拟南芥叶片中翻译后蛋白质修饰的相互作用。
Plant J. 2023 Nov;116(4):1172-1193. doi: 10.1111/tpj.16406. Epub 2023 Jul 31.
5
De novo design of protein structure and function with RFdiffusion.利用 RFdiffusion 从头设计蛋白质结构和功能。
Nature. 2023 Aug;620(7976):1089-1100. doi: 10.1038/s41586-023-06415-8. Epub 2023 Jul 11.
6
The pyrenoid: the eukaryotic CO2-concentrating organelle.淀粉核：真核生物的 CO2 浓缩细胞器。
Plant Cell. 2023 Sep 1;35(9):3236-3259. doi: 10.1093/plcell/koad157.
7
Current Insights into the Redox Regulation Network in Plant Chloroplasts.当前对植物叶绿体氧化还原调控网络的认识。
Plant Cell Physiol. 2023 Jul 17;64(7):704-715. doi: 10.1093/pcp/pcad049.
8
Improving de novo protein binder design with deep learning.利用深度学习改进从头设计的蛋白质结合物。
Nat Commun. 2023 May 6;14(1):2625. doi: 10.1038/s41467-023-38328-5.
9
Chloroplast gene expression: Recent advances and perspectives.叶绿体基因表达：最新进展与展望。
Plant Commun. 2023 Sep 11;4(5):100611. doi: 10.1016/j.xplc.2023.100611. Epub 2023 May 4.
10
Improving crop yield potential: Underlying biological processes and future prospects.提高作物产量潜力：潜在的生物学过程与未来前景。
Food Energy Secur. 2022 Dec 2;12(1):e435. doi: 10.1002/fes3.435. eCollection 2023 Jan.
Zotero 插件