Botanisches Institut der Universität München, Menzinger Strasse 67, D-8000 München 19.
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6244-8. doi: 10.1073/pnas.80.20.6244.
Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, P(fr)) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the P(fr) chromopeptide and the Z,Z,E-configurated phycocyanin chromopeptide behave similarly with regard to spectral and chromatographic properties and reactivity. We present evidence here, obtained by high-resolution (1)H NMR spectroscopy, that both the modified phycocyanobilin chromophore and the phytochrome chromophore obtained directly from P(fr) are 15E-configurated.
色肽是通过对光敏色素(远红吸收形式,P(fr))和藻蓝蛋白的蛋白水解消化制备的。藻蓝胆素肽的发色团为 Z,Z,Z-构型,被修饰为 Z,Z,E-异构发色团。先前已经证明,P(fr)色肽和 Z,Z,E-构型的藻蓝蛋白色肽在光谱和色谱性质以及反应性方面具有相似的行为。我们在这里通过高分辨率(1)H NMR 光谱提供了证据,证明修饰的藻蓝胆素发色团和直接从 P(fr)获得的光敏素发色团都是 15E-构型的。