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本文引用的文献

1
Purification of phytochrome by Affi-Gel Blue chromatography; an effect of lumichrome on purified phytochrome.用Affi-Gel Blue层析法纯化光敏色素;核黄素对纯化的光敏色素的影响。
Anal Biochem. 1981 Oct;117(1):32-9. doi: 10.1016/0003-2697(81)90687-4.
2
Primary photoprocesses of undegraded phytochrome excited with red and blue light at 77 K.77K 下未降解的光敏色素在红光和蓝光激发下的初级光过程。
Biochim Biophys Acta. 1981 Apr 13;635(2):369-82. doi: 10.1016/0005-2728(81)90035-9.
3
Phototransformation and dark reversion of phytochrome in deuterium oxide.氧化氘中光敏色素的光转化和暗逆转
Biochemistry. 1981 Jul 21;20(15):4315-20. doi: 10.1021/bi00518a012.
4
The kinetics of phytochrome conversion.光敏色素转化的动力学
J Biol Chem. 1966 Jul 25;241(14):3395-403.
5
Purification and properties of phytochrome: a chromoprotein regulating plant growth.光敏色素的纯化与性质:一种调节植物生长的色素蛋白
Fed Proc. 1965 Jul-Aug;24(4):863-7.
6
Low-temperature absorption and circular dichroism studies of phytochrome.植物色素的低温吸收与圆二色性研究
Biochemistry. 1972 Oct 24;11(22):4025-31. doi: 10.1021/bi00772a003.
7
Isolation and partial characterization of a chromophore-peptide fragment from pepsin digests of phytochrome.从植物色素的胃蛋白酶消化物中分离出一种发色团 - 肽片段并进行部分特性鉴定。
Biochem Biophys Res Commun. 1971 Dec 17;45(6):1466-73. doi: 10.1016/0006-291x(71)90185-9.
8
The molecular basis of visual excitation.视觉兴奋的分子基础。
Nature. 1968 Aug 24;219(5156):800-7. doi: 10.1038/219800a0.
9
Chromophore content of blue-green algal phycobiliproteins.蓝绿藻藻胆蛋白的发色团含量
J Biol Chem. 1973 Jan 25;248(2):659-62.
10
On the linkages between chromophore and protein in biliproteins, VI[1]. Structure and protein linkage of the phytochrome chromophore.关于胆色素蛋白中发色团与蛋白质的联系,VI[1]。植物色素发色团的结构与蛋白质联系
Hoppe Seylers Z Physiol Chem. 1977 Aug;358(8):1077-9.

光色素生理活性形式(P(fr))的发色团结构。

Chromophore structure of the physiologically active form (P(fr)) of phytochrome.

机构信息

Botanisches Institut der Universität München, Menzinger Strasse 67, D-8000 München 19.

出版信息

Proc Natl Acad Sci U S A. 1983 Oct;80(20):6244-8. doi: 10.1073/pnas.80.20.6244.

DOI:10.1073/pnas.80.20.6244
PMID:16593380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394272/
Abstract

Chromopeptides were prepared by proteolytic digestion of phytochrome (far-red absorbing form, P(fr)) and of phycocyanin. The phycocyanobilin peptide, the chromophore of which is Z,Z,Z-configurated, was modified to the Z,Z,E isomeric chromophore. It has been demonstrated earlier that the P(fr) chromopeptide and the Z,Z,E-configurated phycocyanin chromopeptide behave similarly with regard to spectral and chromatographic properties and reactivity. We present evidence here, obtained by high-resolution (1)H NMR spectroscopy, that both the modified phycocyanobilin chromophore and the phytochrome chromophore obtained directly from P(fr) are 15E-configurated.

摘要

色肽是通过对光敏色素(远红吸收形式,P(fr))和藻蓝蛋白的蛋白水解消化制备的。藻蓝胆素肽的发色团为 Z,Z,Z-构型,被修饰为 Z,Z,E-异构发色团。先前已经证明,P(fr)色肽和 Z,Z,E-构型的藻蓝蛋白色肽在光谱和色谱性质以及反应性方面具有相似的行为。我们在这里通过高分辨率(1)H NMR 光谱提供了证据,证明修饰的藻蓝胆素发色团和直接从 P(fr)获得的光敏素发色团都是 15E-构型的。