Institut für Chemie, Technische Universität Berlin, Sekr. PC 14, Straße des 17. Juni 135, D-10623 Berlin, Germany.
Biomolecules. 2023 Jun 17;13(6):1007. doi: 10.3390/biom13061007.
Phytochromes are biological photoswitches that translate light into physiological functions. Spectroscopic techniques are essential tools for molecular research into these photoreceptors. This review is directed at summarizing how resonance Raman and IR spectroscopy contributed to an understanding of the structure, dynamics, and reaction mechanism of phytochromes, outlining the substantial experimental and theoretical challenges and describing the strategies to master them. It is shown that the potential of the various vibrational spectroscopic techniques can be most efficiently exploited using integral approaches via a combination of theoretical methods as well as other experimental techniques.
光敏色素是将光转化为生理功能的生物光开关。光谱技术是对这些光感受器进行分子研究的重要工具。本综述旨在总结共振拉曼和红外光谱如何促进对光敏色素的结构、动态和反应机制的理解，概述了大量的实验和理论挑战，并描述了掌握这些挑战的策略。结果表明，通过结合理论方法以及其他实验技术，通过综合方法可以最有效地利用各种振动光谱技术的潜力。
