Laboratory of Genetics, University of Wisconsin, Madison, WI 53706.
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7591-5. doi: 10.1073/pnas.80.24.7591.
Transposition of a receptor element (Rs) for the suppressor-mutator (Spm) has generated the first Spm-controlled mutable allele of the bronze-1 (bz) locus in maize (Zea mays L.). In the absence of Spm, bz-m13 conditions full anthocyanin pigmentation in the aleurone but has a markedly decreased level compared to wild type of the gene product, UDPglucose:flavonol 3-O-glucosyltransferase (UFGT; EC 2.4.1.91). This UFGT activity appears to be qualitatively similar to the wild-type enzyme. Throughout endosperm development, the amount of a crossreactive material far exceeds the amount of detectable UFGT activity. This and other observations suggest that this insertion of Rs leads to the production of two polypeptides from the bz-m13 allele.
转座一个受体元件 (Rs) 为抑制突变体 (Spm) 已经在玉米 (Zea mays L.) 中产生了第一个 Spm 控制的青铜 1 (bz) 基因座的可突变等位基因。在没有 Spm 的情况下,bz-m13 使糊粉层完全产生花青素色素,但与基因产物 UDP-葡萄糖:类黄酮 3-O-葡萄糖基转移酶 (UFGT; EC 2.4.1.91) 的野生型相比,其水平明显降低。这种 UFGT 活性似乎在质量上与野生型酶相似。在整个胚乳发育过程中,交叉反应物质的量远远超过可检测 UFGT 活性的量。这和其他观察结果表明,这种 Rs 的插入导致从 bz-m13 等位基因产生两种多肽。