Rubin G M, Spradling A C
Science. 1982 Oct 22;218(4570):348-53. doi: 10.1126/science.6289436.
Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos. Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes.
将外源DNA序列导入果蝇种系。通过将包含野生型玫瑰红基因的染色体DNA片段插入P转座元件构建的玫瑰红转座子（ry1），在20%至50%的注射了玫瑰红突变胚胎中转化了种系细胞。转化体含有一或两个染色体整合的完整ry1拷贝，这些拷贝在后代中稳定遗传。这些转化后的果蝇具有野生型眼色，表明宿主菌株中可见的遗传缺陷可被转移的基因完全且永久地纠正。为证明该方法的通用性，一个不给受体赋予可识别表型的DNA片段也被转移到种系染色体中。
