玉米缩减座位点的控制元件事件。
Controlling-element events at the shrunken locus in maize.
机构信息
Department of Biology, Brookhaven National Laboratory, Upton, NY 11973.
出版信息
Genetics. 1981 May;98(1):143-56. doi: 10.1093/genetics/98.1.143.
Abstract
We have examined insertions of the controlling element Ds at the Shrunken locus of maize. A cDNA probe complementary to a portion of the Shrunken locus mRNA was prepared. This probe recognizes a unique sequence in maize DNA. Using lines carrying derivatives of the same short arm of chromosome 9, we have detected modifications at the nucleic acid level caused by Ds. The changes appear to be large insertions, one of which may be more than 20 kilobase pairs in length. These observations provide a basis for the isolation and molecular characterization of one of the maize controlling elements.
摘要
我们已经研究了控制元件 Ds 在玉米 Shrunken 基因座的插入情况。我们制备了与 Shrunken 基因座 mRNA 部分互补的 cDNA 探针。该探针识别玉米 DNA 中的一个独特序列。利用携带同一 9 号染色体短臂衍生物的系，我们检测到了 Ds 引起的核酸水平上的修饰。这些变化似乎是大的插入，其中一个可能超过 20 千碱基对长。这些观察结果为分离和分子鉴定一个玉米控制元件提供了基础。
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