Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611.
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3726-30. doi: 10.1073/pnas.82.11.3726.
The DNA sequence of a gene (Gmhsp17.5-E) encoding a small heat shock protein of soybean, Glycine max, has been determined. Nuclease S1 mapping of the 5' terminus of the corresponding RNA indicates that the start site for transcription is located 82 bases upstream from the coding region and 24 bases downstream from a "TATA"-like region (-T-T-T-A-A-A-T-A-). The 5' flanking region of Gmhsp17.5-E contains two imperfect dyads that closely resemble regulatory elements present in the promoters of heat-inducible genes of Drosophila. One, positioned 18 bases upstream from the TATA-like region, shows 90% homology to the Drosophila heat shock consensus sequence. The other overlaps an upstream TATA sequence and is located at position -213. Analysis of the derived amino acid sequence indicates that the protein encoded by Gmhsp17.5-E is related structurally to the four small heat shock proteins of Drosophila. This relationship is most evident by comparison of hydropathy profiles; they show conservation of several major hydrophilic and hydrophobic regions, which suggests that these proteins have common structural features.
已确定编码大豆（Glycine max）小热休克蛋白的基因（Gmhsp17.5-E）的 DNA 序列。用核酸酶 S1 对相应 RNA 的 5'末端进行作图表明，转录的起始位点位于编码区上游 82 个碱基处，并且在“TATA”样区域（-T-T-T-A-A-A-T-A-）下游 24 个碱基处。Gmhsp17.5-E 的 5'侧翼区包含两个不完全的二联体，它们与果蝇热诱导基因启动子中存在的调节元件非常相似。一个位于 TATA 样区域上游 18 个碱基处，与果蝇热休克共有序列具有 90%的同源性。另一个与上游 TATA 序列重叠，位于 -213 位。对推导的氨基酸序列的分析表明，由 Gmhsp17.5-E 编码的蛋白质在结构上与果蝇的四种小热休克蛋白有关。通过比较疏水性图谱可以最明显地看出这种关系；它们显示出几个主要亲水性和疏水性区域的保守性，这表明这些蛋白质具有共同的结构特征。
