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一个编码组蛋白变体的海胆组蛋白DNA克隆中普遍存在的和基因特异性的调控5'序列。

Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.

作者信息

Busslinger M, Portmann R, Irminger J C, Birnstiel M L

出版信息

Nucleic Acids Res. 1980 Mar 11;8(5):957-77. doi: 10.1093/nar/8.5.957.

DOI:10.1093/nar/8.5.957
PMID:7443547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC323965/
Abstract

The DNA sequences of the entire structural H4, H3, H2A and H2B genes and of their 5' flanking regions have been determined in the histone DNA clone h19 of the sea urchin Psammechinus miliaris. In clone h19 the polarity of transcription and the relative arrangement of the histone genes is identical to that in clone h22 of the same species. The histone proteins encoded by h19 DNA differ in their primary structure from those encoded by clone h22 and have been compared to histone protein sequences of other sea urchin species as well as other eukaryotes. A comparative analysis of the 5' flanking DNA sequences of the structural histone genes in both clones revealed four ubiquitous sequence motifs; a pentameric element GATCC, followed at short distance by the Hogness box GTATAAATAG, a conserved sequence PyCATTCPu, in or near which the 5' ends of the mRNAs map in h22 DNA and lastly a sequence A, containing the initiation codon. These sequences are also found, sometimes in modified version, in front of other eukaryotic genes transcribed by polymerase II. When prelude sequences of isocoding histone genes in clone h19 and h22 are compared areas of homology are seen to extend beyond the ubiquitous sequence motifs towards the divergent AT-rich spacer and terminate between approximately 140 and 240 nucleotides away from the structural gene. These prelude regions contain quite large conservative sequence blocks which are specific for each type of histone genes.

摘要

已测定了海胆粗疣海胆（Psammechinus miliaris）组蛋白DNA克隆h19中整个结构H4、H3、H2A和H2B基因及其5'侧翼区域的DNA序列。在克隆h19中，转录极性和组蛋白基因的相对排列与同一物种的克隆h22中的相同。由h19 DNA编码的组蛋白在一级结构上与由克隆h22编码的组蛋白不同，并已与其他海胆物种以及其他真核生物的组蛋白序列进行了比较。对两个克隆中结构组蛋白基因的5'侧翼DNA序列进行的比较分析揭示了四个普遍存在的序列基序：一个五聚体元件GATCC，在短距离后跟着霍格内斯盒GTATAAATAG，一个保守序列PyCATTCPu，h22 DNA中mRNA的5'末端定位在该序列内或附近，最后是一个包含起始密码子的序列A。这些序列有时以修饰形式也存在于由聚合酶II转录的其他真核基因之前。当比较克隆h19和h22中同编码组蛋白基因的前导序列时，发现同源区域延伸到普遍存在的序列基序之外，朝向富含AT的发散间隔区，并在距结构基因约140至240个核苷酸之间终止。这些前导区域包含相当大的保守序列块，这些块对每种组蛋白基因都是特异的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8293/323965/099a5adf7e53/nar00422-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8293/323965/2cb7584749b1/nar00422-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8293/323965/099a5adf7e53/nar00422-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8293/323965/2cb7584749b1/nar00422-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8293/323965/099a5adf7e53/nar00422-0045-a.jpg

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