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2
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本文引用的文献

1
Introduction of genetic material into plant cells.遗传物质导入植物细胞。
Science. 1983 Nov 18;222(4625):815-21. doi: 10.1126/science.222.4625.815.
2
Short direct repeats flank the T-DNA on a nopaline Ti plasmid.短直接重复序列侧翼在胭脂碱 Ti 质粒上的 T-DNA。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6322-6. doi: 10.1073/pnas.79.20.6322.
3
Complete nucleotide sequence of a soybean actin gene.大豆肌动蛋白基因的完整核苷酸序列。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1022-6. doi: 10.1073/pnas.79.4.1022.
4
Pattern recognition in genetic sequences.基因序列中的模式识别。
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3041. doi: 10.1073/pnas.76.7.3041.
5
Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.在毛叶冠瘿碱型根瘤中鉴定 TL-DNA 转录本的功能
EMBO J. 1982;1(1):147-52. doi: 10.1002/j.1460-2075.1982.tb01138.x.
6
The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.在毛叶番荔枝冠瘿瘤的 TL-DNA 中，编码了 7 个明确的多聚腺苷酸化转录本。
EMBO J. 1982;1(1):139-46. doi: 10.1002/j.1460-2075.1982.tb01137.x.
7
Eukaryotic promoters?真核生物启动子？
Nature. 1979 May 31;279(5712):376. doi: 10.1038/279376a0.
8
Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models.以农杆菌T-DNA基因作为模型鉴定高等植物核转录本聚腺苷酸化所涉及的序列。
EMBO J. 1983;2(3):419-26. doi: 10.1002/j.1460-2075.1983.tb01439.x.
9
The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria.Ti质粒中T区域的保守部分在细菌中表达四种蛋白质。
EMBO J. 1983;2(3):403-9. doi: 10.1002/j.1460-2075.1983.tb01437.x.
10
The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants.Ti质粒携带的冠瘿碱合成酶基因包含在植物中表达所需的所有信号。
EMBO J. 1983;2(9):1597-603. doi: 10.1002/j.1460-2075.1983.tb01630.x.

根癌土壤杆菌质粒pTiAch5的TL-DNA的完整核苷酸序列。

The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5.

作者信息

Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M, Van Montagu M, Schell J

出版信息

EMBO J. 1984 Apr;3(4):835-46. doi: 10.1002/j.1460-2075.1984.tb01894.x.

DOI:10.1002/j.1460-2075.1984.tb01894.x
PMID:6327292
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557436/
Abstract

We have determined the complete primary structure (13 637 bp) of the TL-region of Agrobacterium tumefaciens octopine plasmid pTiAch5 . This sequence comprises two small direct repeats which flank the TL-region at each extremity and are involved in the transfer and/or integration of this DNA segment in plants. TL-DNA specifies eight open-reading frames corresponding to experimentally identified transcripts in crown gall tumor tissue. The eight coding regions are not interrupted by intervening sequences and are separated from each other by AT-rich regions. Potential transcriptional control signals upstream of the 5' and 3' ends of all the transcribed regions resemble typical eukaryotic signals: (i) transcriptional initiation signals ('TATA' or Goldberg- Hogness box) are present upstream to the presumed translational start codons; (ii) ' CCAAT ' sequences are present upstream of the proposed 'TATA' box; (iii) polyadenylation signals are present in the 3'-untranslated regions. Furthermore, no Shine-Dalgarno sequences are present upstream of the presumed translational start codons.

摘要

我们已经确定了根癌土壤杆菌章鱼碱型质粒pTiAch5的TL区域的完整一级结构（13637碱基对）。该序列包含两个小的正向重复序列，它们位于TL区域的两端，参与该DNA片段在植物中的转移和/或整合。TL-DNA指定了八个开放阅读框，对应于在冠瘿瘤组织中通过实验鉴定的转录本。这八个编码区域没有被间隔序列打断，并且彼此之间由富含AT的区域隔开。所有转录区域5'和3'末端上游的潜在转录控制信号类似于典型的真核信号：（i）转录起始信号（“TATA”或戈德堡-霍格尼斯盒）存在于假定的翻译起始密码子上游；（ii）“CCAAT”序列存在于提议的“TATA”盒上游；（iii）聚腺苷酸化信号存在于3'非翻译区域。此外，在假定的翻译起始密码子上游不存在Shine-Dalgarno序列。