Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M, Van Montagu M, Schell J
EMBO J. 1984 Apr;3(4):835-46. doi: 10.1002/j.1460-2075.1984.tb01894.x.
We have determined the complete primary structure (13 637 bp) of the TL-region of Agrobacterium tumefaciens octopine plasmid pTiAch5 . This sequence comprises two small direct repeats which flank the TL-region at each extremity and are involved in the transfer and/or integration of this DNA segment in plants. TL-DNA specifies eight open-reading frames corresponding to experimentally identified transcripts in crown gall tumor tissue. The eight coding regions are not interrupted by intervening sequences and are separated from each other by AT-rich regions. Potential transcriptional control signals upstream of the 5' and 3' ends of all the transcribed regions resemble typical eukaryotic signals: (i) transcriptional initiation signals ('TATA' or Goldberg- Hogness box) are present upstream to the presumed translational start codons; (ii) ' CCAAT ' sequences are present upstream of the proposed 'TATA' box; (iii) polyadenylation signals are present in the 3'-untranslated regions. Furthermore, no Shine-Dalgarno sequences are present upstream of the presumed translational start codons.
我们已经确定了根癌土壤杆菌章鱼碱型质粒pTiAch5的TL区域的完整一级结构（13637碱基对）。该序列包含两个小的正向重复序列，它们位于TL区域的两端，参与该DNA片段在植物中的转移和/或整合。TL-DNA指定了八个开放阅读框，对应于在冠瘿瘤组织中通过实验鉴定的转录本。这八个编码区域没有被间隔序列打断，并且彼此之间由富含AT的区域隔开。所有转录区域5'和3'末端上游的潜在转录控制信号类似于典型的真核信号：（i）转录起始信号（“TATA”或戈德堡-霍格尼斯盒）存在于假定的翻译起始密码子上游；（ii）“CCAAT”序列存在于提议的“TATA”盒上游；（iii）聚腺苷酸化信号存在于3'非翻译区域。此外，在假定的翻译起始密码子上游不存在Shine-Dalgarno序列。
