丁香假单胞菌细胞分裂素生物合成基因的核苷酸序列与表达：与根癌土壤杆菌tmr和tzs基因座的同源性
Nucleotide sequence and expression of a Pseudomonas savastanoi cytokinin biosynthetic gene: homology with Agrobacterium tumefaciens tmr and tzs loci.
作者信息
Powell G K, Morris R O
出版信息
Nucleic Acids Res. 1986 Mar 25;14(6):2555-65. doi: 10.1093/nar/14.6.2555.
The nucleotide sequence of a Pseudomonas trans-zeatin producing gene (ptz) from the pCK1 plasmid of Pseudomonas syringae pv. savastanoi strain 1006 has been determined. This gene confers upon E. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respective N9-ribosyl derivatives. Sequence analysis indicates an open reading frame encoding a protein of 234 amino acids with a molecular weight of 26,816. Significant sequence homology is found between ptz and both the tzs and tmr genes from Agrobacterium tumefaciens. The results suggest a close relationship between the cytokinin biosynthetic pathways in P. savastanoi and A. tumefaciens.
已测定了丁香假单胞菌丁香致病变种 savastanoi 菌株 1006 的 pCK1 质粒中一个产生反式玉米素的假单胞菌基因（ptz）的核苷酸序列。该基因赋予大肠杆菌合成和分泌多种细胞分裂素的能力，包括反式玉米素、异戊烯腺嘌呤及其各自的 N9 - 核糖基衍生物。序列分析表明有一个开放阅读框，编码一个由 234 个氨基酸组成、分子量为 26,816 的蛋白质。在 ptz 与根癌土壤杆菌的 tzs 和 tmr 基因之间发现了显著的序列同源性。结果表明 savastanoi 假单胞菌和根癌土壤杆菌中细胞分裂素生物合成途径之间存在密切关系。
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