Ullah Uzair, Buttar Zeeshan Ali, Shalmani Abdullah, Muhammad Izhar, Ud-Din Aziz, Ali Hamid
Department of Biotechnology and Genetic Engineering, University Mansehra, Dhodial, Pakistan.
The Collaborative Innovation Center for Grain Crops, Henan Agricultural University, Zhengzhou, China.
Open Life Sci. 2022 May 18;17(1):544-562. doi: 10.1515/biol-2022-0051. eCollection 2022.
The -like plant-specific transcription factor has a prominent role in plant development and growth through cell division and differential activities. However, little information is available about the gene family in L. Herein, we identified 37 and 11 genes in the wheat and rice genome databases, respectively. The phylogeny of the CPP protein-like family members was further divided into five subfamilies based on structural similarities and phenotypic functional diversities. The expression analysis showed that genes are highly expressed in some tissues, such as shoot apex, shoot, leaf, leaf sheath, and microspore. Furthermore, the qRT-PCR found higher expression for gene family members in leaf, leaf blade, young spike, mature spike, and differential expression patterns under abiotic stresses, including heat, drought, salt, and hormonal treatment, such as indole acetic acid and 1-aminocyclopropane-1 carboxylic acid. We found that gene family members are mostly located in the nucleus after infiltrating the and into tobacco leaves. The overexpression of the gene revealed that the gene positively regulates the germanium, shoot, and root activities in . The -overexpressed plants showed less anti-oxidative sensitivity under drought stress conditions. These results demonstrated that TaCPP5-1D protein has a crucial contribution by interacting with TaCPP11-3B protein in maintaining stress homeostasis under the natural and unfavorable environmental conditions for growth, development, and stress resistance activities. Therefore, this study could be used as pioneer knowledge to further investigate the function of genes in plant growth and development.
类植物特异性转录因子通过细胞分裂和差异活动在植物发育和生长中发挥着重要作用。然而，关于L.中基因家族的信息却很少。在此，我们分别在小麦和水稻基因组数据库中鉴定出37个和11个基因。基于结构相似性和表型功能多样性，CPP蛋白样家族成员的系统发育进一步分为五个亚家族。表达分析表明，基因在一些组织中高表达，如茎尖、茎、叶、叶鞘和小孢子。此外，qRT-PCR发现基因家族成员在叶、叶片、幼穗、成熟穗中表达较高，并且在非生物胁迫下，包括热、干旱、盐以及激素处理（如吲哚乙酸和1-氨基环丙烷-1-羧酸）下呈现差异表达模式。我们发现将和渗入烟草叶片后，基因家族成员大多位于细胞核中。基因的过表达表明该基因正向调控中的锗、茎和根的活性。过表达的植物在干旱胁迫条件下表现出较低的抗氧化敏感性。这些结果表明，TaCPP5-1D蛋白通过与TaCPP11-3B蛋白相互作用，在自然和不利于生长、发育及抗逆活动的环境条件下维持胁迫稳态方面发挥着关键作用。因此，本研究可为进一步探究基因在植物生长发育中的功能提供先驱性知识。
