表达1-脱氧-D-阿拉伯庚酮糖酸-7-磷酸（DAHP）合酶反义构建体的转基因马铃薯植株中，伤口诱导DAHP合酶受损及茎发育改变
Impaired Wound Induction of 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) Synthase and Altered Stem Development in Transgenic Potato Plants Expressing a DAHP Synthase Antisense Construct.
作者信息
Jones J. D., Henstrand J. M., Handa A. K., Herrmann K. M., Weller S. C.
机构信息
Departments of Horticulture (J.D.J., J.M.H., A.K.H., S.C.W.) and Biochemistry (K.M.H.), Purdue University, West Lafayette, Indiana 47907-1165.
出版信息
Plant Physiol. 1995 Aug;108(4):1413-1421. doi: 10.1104/pp.108.4.1413.
Abstract
Potato (Solanum tuberosum L.) cells were transformed with an antisense DNA construct encoding part of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15), the first enzyme of the shikimate pathway, to examine the role(s) of this protein in plant growth and development. Chimeric DNA constructs contained the transcript start site, the first exon, and part of the first intron of the shkA gene in antisense or sense orientations under the control of the cauliflower mosaic virus 35S promoter. Some, but not all, of the transgenic plants expressing antisense DAHP synthase RNA showed reduced levels of wound-induced DAHP synthase enzyme activity, polypeptide, and mRNA 12 and 24 h after wounding. No alteration in the wound induction of DAHP synthase gene expression was observed in transgenic potato tubers containing the chimeric sense construct. Reduced steady-state levels of DAHP synthase mRNA were observed in stem and shoot tip tissue. Some plants with the chimeric antisense construct had reduced stem length, stem diameter, and reduced stem lignification.
摘要
用编码莽草酸途径的第一个酶3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸（DAHP）合酶（EC 4.1.2.15）部分序列的反义DNA构建体转化马铃薯（Solanum tuberosum L.）细胞，以研究该蛋白在植物生长发育中的作用。嵌合DNA构建体包含转录起始位点、第一个外显子以及shkA基因第一个内含子的部分序列，它们以反义或正义方向置于花椰菜花叶病毒35S启动子的控制之下。一些（但并非全部）表达反义DAHP合酶RNA的转基因植物在受伤后12小时和24小时，伤口诱导的DAHP合酶活性、多肽和mRNA水平降低。在含有嵌合正义构建体的转基因马铃薯块茎中，未观察到DAHP合酶基因表达的伤口诱导有任何改变。在茎和茎尖组织中观察到DAHP合酶mRNA的稳态水平降低。一些带有嵌合反义构建体的植物茎长、茎直径减小，茎木质化程度降低。
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2
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