Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, Brazil.
Laboratório de Toxinologia Aplicada, Instituto Butantan, São Paulo 05503-900, Brazil.
Int J Mol Sci. 2022 Oct 1;23(19):11603. doi: 10.3390/ijms231911603.
Initially, natural antisense transcripts (NATs, natRNAs, or asRNAs) were considered repressors; however, their functions in gene regulation are diverse. Positive, negative, or neutral correlations to the cognate gene expression have been noted. Although the first studies were published about 50 years ago, there is still much to be investigated regarding antisense transcripts in plants. A systematic review of scientific publications available in the Web of Science databases was conducted to contextualize how the studying of antisense transcripts has been addressed. Studies were classified considering three categories: "Natural antisense" (208), artificial antisense used in "Genetic Engineering" (797), or "Natural antisense and Genetic Engineering"-related publications (96). A similar string was used for a systematic search in the NCBI Gene database. Of the 1132 antisense sequences found for plants, only 0.8% were cited in PubMed and had antisense information confirmed. This value was the lowest when compared to fungi (2.9%), bacteria (2.3%), and mice (54.1%). Finally, we present an update for the cis-NATs identified in spp. Of the 1413 antisense transcripts found in different experiments, 25 showed concordant expressions, 22 were discordant, 1264 did not correlate with the cognate genes, and 102 presented variable results depending on the experiment.
最初，天然反义转录本（NATs、natRNAs 或 asRNAs）被认为是抑制剂；然而，它们在基因调控中的功能是多样的。已经注意到与同源基因表达呈正相关、负相关或中性相关。尽管第一批研究报告是在大约 50 年前发表的，但关于植物中的反义转录本仍有许多需要研究。对 Web of Science 数据库中可用的科学出版物进行了系统审查，以了解研究反义转录本的情况。研究根据三个类别进行分类：“天然反义”（208）、用于“基因工程”的人工反义（797）或“天然反义与基因工程”相关出版物（96）。在 NCBI Gene 数据库中使用了类似的字符串进行系统搜索。在为植物找到的 1132 条反义序列中，只有 0.8%在 PubMed 中被引用并确认具有反义信息。与真菌（2.9%）、细菌（2.3%）和老鼠（54.1%）相比，这个值最低。最后，我们介绍了在 spp.中鉴定的顺式-NATs 的更新。在不同实验中发现的 1413 条反义转录本中，有 25 条表现出一致的表达，22 条表达不一致，1264 条与同源基因不相关，102 条根据实验结果呈现出不同的结果。
