通过抗体检测法评估牛口蹄疫疫苗的效力
Potency assessment of foot-and-mouth disease vaccines in cattle by means of antibody assays.
作者信息
Amadori M, Archetti I L, Tollis M, Buonavoglia C, Panina G F
机构信息
Istituto Zooprofilattico Sperimentale della Lombardia e dell 'Emilia, Brescia, Italy.
出版信息
Biologicals. 1991 Jul;19(3):191-6. doi: 10.1016/1045-1056(91)90034-h.
A tendency has emerged for some years to replace the challenge infection of cattle for the assessment of foot-and-mouth disease (FMD) vaccine potency. This can be actually evaluated by means of antibody assays on cattle sera, at about 3/4 weeks after the vaccination. Serological results can be worked out as single titres (to be compared with a pre-determined threshold level) or as mean antibody titres induced by different vaccine dilutions. However, the assessment of FMDV-specific antibody titres would not fully depict the extent and the efficacy of the immune response of cattle; moreover, the antibody response would not be proportional if potent vaccines are used (greater than or equal to 10-12 PD50). Thus, a particular approach is suggested for the serological procedures, which enable credible estimates of potent FMD vaccines to be formulated.
多年来，出现了一种趋势，即取代通过用牛进行攻毒感染来评估口蹄疫（FMD）疫苗效力的方法。实际上，可以在接种疫苗后约3/4周，通过对牛血清进行抗体检测来评估。血清学结果可以作为单个滴度（与预先确定的阈值水平进行比较）或作为不同疫苗稀释度诱导的平均抗体滴度得出。然而，对口蹄疫病毒特异性抗体滴度的评估并不能完全描述牛免疫反应的程度和效力；此外，如果使用强效疫苗（大于或等于10-12 PD50），抗体反应将不成比例。因此，建议采用一种特定的血清学检测方法，以便能够制定出对强效口蹄疫疫苗的可靠评估。
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