Wu Maoqing, Yin Gang, Zhao Xin, Ji Chaoneng, Gu Shaohua, Tang Rong, Dong Hai, Xie Yi, Mao Yumin
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
Int J Mol Med. 2006 May;17(5):749-54.
As an atypical member of the Rab family, Rab24 has several attributes distinguishing this protein from the other members. Based on the yeast two-hybrid system, interaction between human RAB24 and two proteins, cyclophilin A (CyP-A) and gamma-aminobutyric acid type A receptor-associated protein (GABARAP), was detected and identified in COS-7 cells. Interestingly, RAB24 is predominantly localized in the nuclei of COS-7 cells, which is different from previous reports using other cell lines. RAB24 (D123I) can trigger the accumulation of intracellular inclusions, with small quantities of intranuclear inclusions in some cells. The GTPase activity of RAB24 and its two mutants was detected.
作为Rab家族的一个非典型成员，Rab24具有一些使其与其他成员不同的特性。基于酵母双杂交系统，在COS-7细胞中检测并鉴定出人类RAB24与两种蛋白，亲环素A（CyP-A）和γ-氨基丁酸A型受体相关蛋白（GABARAP）之间的相互作用。有趣的是，RAB24主要定位于COS-7细胞的细胞核中，这与之前使用其他细胞系的报道不同。RAB24（D123I）可引发细胞内包涵体的积累，在一些细胞中有少量核内包涵体。检测了RAB24及其两个突变体的GTPase活性。
