珊瑚相关细菌群落的荧光原位杂交和光谱成像
Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities.
作者信息
Ainsworth T D, Fine M, Blackall L L, Hoegh-Guldberg O
机构信息
Centre for Marine Studies, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia.
出版信息
Appl Environ Microbiol. 2006 Apr;72(4):3016-20. doi: 10.1128/AEM.72.4.3016-3020.2006.
Abstract
Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.
摘要
微生物群落对珊瑚礁群落的功能起着重要作用。然而，珊瑚组织和内共生体的广泛自发荧光限制了标准荧光原位杂交（FISH）技术在鉴定与珊瑚相关细菌群落中的应用。本研究通过结合FISH和光谱成像克服了这些限制。
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