Wohlsen T, Bates J, Vesey G, Robinson W A, Katouli M
Public Health Microbiology, Queensland Health Scientific Services, Coopers Plains, Brisbane, Qld, Australia.
Lett Appl Microbiol. 2006 Apr;42(4):350-6. doi: 10.1111/j.1472-765X.2006.01854.x.
To use BioBall cultures as a precise reference standard to evaluate methods for enumeration of Escherichia coli and other coliform bacteria in water samples.
Eight methods were evaluated including membrane filtration, standard plate count (pour and spread plate methods), defined substrate technology methods (Colilert and Colisure), the most probable number method and the Petrifilm disposable plate method. Escherichia coli and Enterobacter aerogenes BioBall cultures containing 30 organisms each were used. All tests were performed using 10 replicates. The mean recovery of both bacteria varied with the different methods employed.
The best and most consistent results were obtained with Petrifilm and the pour plate method. Other methods either yielded a low recovery or showed significantly high variability between replicates.
The BioBall is a very suitable quality control tool for evaluating the efficiency of methods for bacterial enumeration in water samples.
使用生物球培养物作为精确的参考标准，以评估水样中大肠杆菌和其他大肠菌群细菌的计数方法。
评估了八种方法，包括膜过滤法、标准平板计数法（倾注平板法和涂布平板法）、特定底物技术方法（Colilert和Colisure）、最可能数法和Petrifilm一次性平板法。使用了分别含有30个菌体的大肠杆菌和气杆菌生物球培养物。所有测试均进行10次重复。两种细菌的平均回收率因所采用的不同方法而异。
Petrifilm和倾注平板法获得了最佳且最一致的结果。其他方法要么回收率低，要么重复之间显示出显著的高变异性。
生物球是评估水样中细菌计数方法效率的非常合适的质量控制工具。
