Lima Ângela, Muzny Christina A, Cerca Nuno
Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), Centre of Biological Engineering (CEB), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, AL 35233, USA.
Microorganisms. 2024 Jan 5;12(1):114. doi: 10.3390/microorganisms12010114.
species are the main colonizers of the vaginal microbiota in healthy women. Their absolute quantification by culture-based methods is limited due to their fastidious growth. Flow cytometry can quantify the bacterial concentration of these bacteria but requires the acquisition of expensive equipment. More affordable non-culturable methods, such as fluorescence microscopy, are hampered by the small size of the bacteria. Herein, we developed an indirect fluorescence microscopy method to determine vaginal lactobacilli concentration by determining the correlation between surface area bacterial measurement and initial concentration of an easily cultivable bacterium () and applying it to lactobacilli fluorescence microscopy counts. In addition, vaginal lactobacilli were quantified by colony-forming units and flow cytometry in order to compare these results with the indirect method results. The colony-forming-unit values were lower than the results obtained from the other two techniques, while flow cytometry and fluorescence microscopy results agreed. Thus, our developed method was able to accurately quantify vaginal lactobacilli.
在健康女性中，[具体菌种]是阴道微生物群的主要定植菌。由于其生长苛求，基于培养的方法对其进行绝对定量存在局限性。流式细胞术可对这些细菌的浓度进行定量，但需要购置昂贵的设备。更经济实惠的非培养方法，如荧光显微镜检查，因细菌体积小而受到阻碍。在此，我们开发了一种间接荧光显微镜方法，通过确定细菌表面积测量值与一种易于培养的细菌（[具体细菌名称]）初始浓度之间的相关性，并将其应用于乳酸杆菌荧光显微镜计数，来测定阴道乳酸杆菌浓度。此外，通过菌落形成单位和流式细胞术对阴道乳酸杆菌进行定量，以便将这些结果与间接方法的结果进行比较。菌落形成单位值低于其他两种技术获得的结果，而流式细胞术和荧光显微镜检查结果一致。因此，我们开发的方法能够准确地对阴道乳酸杆菌进行定量。
