Chen Hua-jie, Yu Ri-an, Wu Zhi-gang, Xia Tao, Yang Cheng-feng, Yang Ke-di, Chen Xue-min
Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Jan;24(1):35-8.
To study the effects of sodium selenite on expression of telomerase reverse transcriptase mRNA, c-Myc and p53 induced by cadmium chloride in rat liver.
Male SD rats were divided randomly into 6 groups, each group had 5 animals. The groups comprised the control group, Se group (5 micromol/kg sodium selenite), 5 micromol/kg cadmium chloride group, 10 micromol/kg cadmium chloride group, Se (5 micromol/kg sodium selenite) + 5 micromol/kg cadmium chloride group, Se (5 micromol/kg sodium selenite) + 10 micromol/kg cadmium chloride group. After 48 hours of the first injection, the expression of TERT mRNA was measured with RT-PCR and c-Myc, and p53 proteins were measured by immunohistochemistry method.
Compared with control group, the expression of TERT was increased in 5 micromol/kg Cd group and 10 micromol/kg Cd group, c-Myc protein was increased in 10 micromol/kg Cd group, and the expression of p53 protein was increased in 5 micromol/kg group and 10 micromol/kg Cd group. TERT expression in Se + 10 micromol/kg Cd group was lower than that of 10 micromol/kg Cd group significantly. c-Myc protein was decreased in Se + 10 micromol/kg Cd group compared with 10 micromol/kg Cd group. p53 protein of Se + 5 micromol/kg Cd group and Se + 10 micromol/kg Cd group were decreased significantly compared with 5 micromol/kg Cd group and 10 micromol/kg Cd group respectively.
The cadmium at the doses of between 5 and 10 micromol/kg can activate TERT and up-regulate c-Myc and p53 proteins. The selenium at the dose of 5 micromol/kg has the antagonistic effect on expression of TERT, c-Myc and p53 induced by cadmium in rat liver.
研究亚硒酸钠对氯化镉诱导的大鼠肝脏端粒酶逆转录酶mRNA、c-Myc和p53表达的影响。
雄性SD大鼠随机分为6组，每组5只。分组包括对照组、硒组（5微摩尔/千克亚硒酸钠）、5微摩尔/千克氯化镉组、10微摩尔/千克氯化镉组、硒（5微摩尔/千克亚硒酸钠）+5微摩尔/千克氯化镉组、硒（5微摩尔/千克亚硒酸钠）+10微摩尔/千克氯化镉组。首次注射48小时后，采用逆转录聚合酶链反应（RT-PCR）检测端粒酶逆转录酶（TERT）mRNA的表达，采用免疫组织化学方法检测c-Myc和p53蛋白。
与对照组相比，5微摩尔/千克镉组和10微摩尔/千克镉组TERT表达增加，10微摩尔/千克镉组c-Myc蛋白增加，5微摩尔/千克组和10微摩尔/千克镉组p53蛋白表达增加。硒+10微摩尔/千克镉组TERT表达明显低于10微摩尔/千克镉组。与10微摩尔/千克镉组相比，硒+10微摩尔/千克镉组c-Myc蛋白减少。硒+5微摩尔/千克镉组和硒+10微摩尔/千克镉组p53蛋白分别与5微摩尔/千克镉组和10微摩尔/千克镉组相比明显减少。
5至10微摩尔/千克剂量的镉可激活TERT并上调c-Myc和p53蛋白。5微摩尔/千克剂量的硒对镉诱导的大鼠肝脏TERT、c-Myc和p53表达具有拮抗作用。
