Hampton G M, Howe C, Leuteritz G, Thomas H, Bodmer W F, Solomon E, Ballhausen W G
Director's Laboratory, Imperial Cancer Research Fund, London, UK.
Hum Genet. 1991 Nov;88(1):112-4. doi: 10.1007/BF00204940.
A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.
