负载中药的固体脂质纳米粒的制备与表征

Preparation and characterization of solid lipid nanoparticles loaded traditional Chinese medicine.

作者信息

Li Yingchao, Dong Lei, Jia Ai, Chang Xinming, Xue Hui

机构信息

Department of Gastroenterology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, PR China.

出版信息

Int J Biol Macromol. 2006 May 30;38(3-5):296-9. doi: 10.1016/j.ijbiomac.2006.03.006. Epub 2006 Mar 10.

DOI:10.1016/j.ijbiomac.2006.03.006

PMID:16600362

Abstract

Ultrasonication was employed to prepare solid lipid nanoparticles (SLN). The model traditional Chinese medicine, tetrandrine (TET), was incorporated into SLN. The TET-loaded SLN (TET-SLN) were spherical in the photograph of transmission electron microscope (TEM). The particle size measured by laser diffraction (LD) was found to be 157.3+/-8.2nm. Zeta potential analyzer suggested the zeta potential of TET-SLN was -29.36+/-3.68mV in distilled water. The entrapment efficiency (EE%) was determined with the sephadex gel chromatogram and high-performance liquid chromatogram (HPLC), and up to 90.59% of TET was incorporated. Stability evaluation showed relatively long-term stability with only slight particle growth (P>0.05) after storage at room temperature for 4 weeks. Therefore, ultrasonication is demonstrated to be a simple, available and effective method to prepare high quality SLN loaded traditional Chinese medicine.

摘要

采用超声法制备固体脂质纳米粒（SLN）。将中药模型药物粉防己碱（TET）载入SLN中。透射电子显微镜（TEM）照片显示载药SLN（TET-SLN）呈球形。通过激光衍射（LD）测得粒径为157.3±8.2nm。ζ电位分析仪显示，TET-SLN在蒸馏水中的ζ电位为-29.36±3.68mV。采用葡聚糖凝胶色谱和高效液相色谱（HPLC）测定包封率（EE%），TET的包封率高达90.59%。稳定性评价表明，室温储存4周后，TET-SLN具有相对长期的稳定性，仅有轻微的颗粒生长（P>0.05）。因此，超声法是一种制备高质量载中药SLN的简单、可行且有效的方法。