Petrásek Jan, Mravec Jozef, Bouchard Rodolphe, Blakeslee Joshua J, Abas Melinda, Seifertová Daniela, Wisniewska Justyna, Tadele Zerihun, Kubes Martin, Covanová Milada, Dhonukshe Pankaj, Skupa Petr, Benková Eva, Perry Lucie, Krecek Pavel, Lee Ok Ran, Fink Gerald R, Geisler Markus, Murphy Angus S, Luschnig Christian, Zazímalová Eva, Friml Jirí
Institute of Experimental Botany, the Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic.
Science. 2006 May 12;312(5775):914-8. doi: 10.1126/science.1123542. Epub 2006 Apr 6.
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
植物激素生长素的细胞间流动支撑着植物的多个发育过程。植物特有的pin形成（PIN）蛋白和几种磷酸糖蛋白（PGP）转运蛋白是生长素运输相关发育的关键因素，但PINs的分子功能仍不清楚。在这里，我们表明，PINs介导生长素从哺乳动物和酵母细胞中流出，而无需其他植物特有的因子。条件性功能获得等位基因以及对拟南芥和烟草培养细胞中生长素积累的定量测量表明，PINs在生长素流出中的作用不同于PGP，是限速的，对生长素具有特异性，并且对生长素运输抑制剂敏感。这表明PINs直接参与催化细胞生长素流出。
