重组杆状病毒表达的猪传染性胃肠炎病毒全序列及无锚定刺突糖蛋白S的加工与抗原性
Processing and antigenicity of entire and anchor-free spike glycoprotein S of coronavirus TGEV expressed by recombinant baculovirus.
作者信息
Godet M, Rasschaert D, Laude H
机构信息
Laboratoire de Virologie et Immunologie Moléculaires, Institute National de la Recherche Agronomique, Jouy-en-Josas, France.
出版信息
Virology. 1991 Dec;185(2):732-40. doi: 10.1016/0042-6822(91)90544-l.
Abstract
The S gene of transmissible gastroenteritis virus (TGEV) was inserted into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV) using the transfer plasmid pVL941. Infection of Sf9 insect cells with the recombinant virus resulted in the synthesis of a 175K polypeptide which was able to trimerize and was transported to the cell surface as is the authentic TGEV S protein. Despite the lack of complete carbohydrate processing, the recombinant S protein exhibited antigenic properties similar to TGEV S and induced high levels of neutralizing antibodies in immunized rats. Engineering a deletion (70 amino acids) into the carboxy-terminus containing the membrane anchor of the polypeptide allowed its secretion. The oligomerization process and the antigenic profile of the anchor-free S protein were shown to be partially altered.
摘要
利用转移质粒pVL941将传染性胃肠炎病毒（TGEV）的S基因插入苜蓿银纹夜蛾核型多角体病毒（AcNPV）的基因组中。用重组病毒感染Sf9昆虫细胞后，产生了一种175K的多肽，该多肽能够三聚化，并像天然TGEV S蛋白一样转运到细胞表面。尽管缺乏完整的碳水化合物加工过程，但重组S蛋白仍表现出与TGEV S相似的抗原特性，并在免疫大鼠中诱导产生高水平的中和抗体。在含有该多肽膜锚定区的羧基末端设计一个缺失（70个氨基酸），使其能够分泌。结果表明，无锚定区S蛋白的寡聚化过程和抗原特性发生了部分改变。
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