微量注射的柯萨奇B1病毒在人喉表皮样癌细胞（HEp-2细胞）中不复制。
Microinjected Coxsackie B1 virus does not replicate in HEp-2 cells.
作者信息
Modalsli K R, Bukholm G, Mikalsen S O, Degré M
机构信息
Kapt. W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway.
出版信息
Virology. 1991 Dec;185(2):888-90. doi: 10.1016/0042-6822(91)90566-t.
PMID:1660207
Abstract
Coxsackie B1 virus did not replicate when microinjected into HEp-2 cells. Replication was assayed by production of infectious virus particles, synthesis of viral RNA, and lysis of cells. The same virus preparation initiated replication when it was inoculated into HEp-2 cells similarly to that by microinjected purified coxsackie B1 virus RNA.
摘要
柯萨奇B1病毒显微注射到HEp - 2细胞后未进行复制。通过感染性病毒颗粒的产生、病毒RNA的合成以及细胞裂解来检测复制情况。当将相同的病毒制剂接种到HEp - 2细胞中时，其引发的复制情况与显微注射纯化的柯萨奇B1病毒RNA时类似。
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